STUDIES OF HUMAN DIPLOID FIBROBLAST GROWTH .1. RESPONSES OF NORMAL AND HYPOPITUITARY CELLS TO FIBROBLAST GROWTH-FACTOR, INSULIN, AND SERUM

被引：14

作者：

JONES, KL ^{[1
]}

ADDISON, J ^{[1
]}

机构：

[1] UNIV CALIF SAN DIEGO, SCH MED, DEPT PEDIAT, LA JOLLA, CA 92093 USA

来源：

JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM | 1976年 / 43卷 / 04期

关键词：

D O I：

10.1210/jcem-43-4-721

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

The growth stimulating activity of bovine insulin, fibroblast growth factor (FGF) and fetal bovine serum (FBS) was assayed in diploid human fibroblasts from normal and idiopathic hypopituitary donors. All 3 factors stimulated DNA synthesis in cells arrested by serum starvation. FGF was active at concentrations as low as 5 ng/ml with maximum effect at 100 ng/ml. FGF stimulated DNA synthesis at lower concentrations than did insulin and also produced a greater maximum response. Only serum was capable of supporting cell division and growth, but FGF accelerated this growth rate when it was added to serum-containing medium. Hydrocortisone, actinomycin D and cycloheximide inhibit FGF stimulation. There was no significant difference between fibroblasts from normal and hypopituitary donors.

引用

页码：721 / 729

页数：9

共 20 条

[1]

CUNNINGHAM D D, 1974, P105

[2] MECHANISM OF REGULATION OF FIBROBLASTIC CELL REPLICATION .3. CENTRAL ROLE FOR NUTRIENT LIMITATION - CONDITIONING EFFECT DUE TO SERUM IMPRINTING OF CELL RESPONSE [J].

ELLEM, KAO ;

MIRONESCU, S .

EXPERIMENTAL CELL RESEARCH, 1974, 88 (01) :175-187

[3] STIMULATION OF DIVISION OF SPARSE AND CONFLUENT 3T3 CELL-POPULATIONS BY A FIBROBLAST GROWTH-FACTOR, DEXAMETHASONE, AND INSULIN [J].

GOSPODAROWICZ, D ;

MORAN, JS .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1974, 71 (11) :4584-4588

[4] LOCALIZATION OF A FIBROBLAST GROWTH-FACTOR AND ITS EFFECT ALONE AND WITH HYDROCORTISONE ON 3T3 CELL-GROWTH [J].

GOSPODAROWICZ, D .

NATURE, 1974, 249 (5453) :123-127

[5]

GOSPODAROWICZ D, 1975, J BIOL CHEM, V250, P2515

[6] STIMULATION OF DIVISION OF Y1 ADRENAL-CELLS BY A GROWTH-FACTOR ISOLATED FROM BOVINE PITUITARY-GLANDS [J].

GOSPODAROWICZ, D ;

HANDLEY, HH .

ENDOCRINOLOGY, 1975, 97 (01) :102-107

[7] MITOGENIC EFFECT OF FIBROBLAST GROWTH-FACTOR ON EARLY PASSAGE CULTURES OF HUMAN AND MURINE FIBROBLASTS [J].

GOSPODAROWICZ, D ;

MORAN, JS .

JOURNAL OF CELL BIOLOGY, 1975, 66 (02) :451-457

[8] PLEIOTYPIC RESPONSE [J].

HERSHKO, A ;

MAMONT, P ;

SHIELDS, R ;

TOMKINS, GM .

NATURE-NEW BIOLOGY, 1971, 232 (33) :206-+

[9] EPIDERMAL GROWTH-FACTOR - RECEPTORS IN HUMAN FIBROBLASTS AND MODULATION OF ACTION BY CHOLERA TOXIN [J].

HOLLENBERG, MD ;

CUATRECASAS, P .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1973, 70 (10) :2964-2968

[10] CONTROL OF INITIATION OF DNA-SYNTHESIS IN 3T3 CELLS - SERUM FACTORS [J].

HOLLEY, RW ;

KIERNAN, JA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1974, 71 (07) :2908-2911

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