THE PROJECTION STRUCTURE OF PERFRINGOLYSIN-O (CLOSTRIDIUM-PERFRINGENS OMICRON-TOXIN)

被引：72

作者：

OLOFSSON, A ^{[1
]}

HEBERT, H ^{[1
]}

THELESTAM, M ^{[1
]}

机构：

[1] KAROLINSKA INST,DEPT BACTERIOL,S-10401 STOCKHOLM 60,SWEDEN

来源：

FEBS LETTERS | 1993年 / 319卷 / 1-2期

关键词：

PERFRINGOLYSIN-O; ELECTRON MICROSCOPY; IMAGE PROCESSING;

D O I：

10.1016/0014-5793(93)80050-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The cytolysin Perfringolysin O was applied to lipid layers and the obtained ring-shaped oligomers analyzed by electron microscopy and image processing. The final result shows the periodic repeat of 2.4 nm along the outer rim of the ring. The asymmetric protein unit, corresponding to one monomer, spans the ring from the convex to the concave surface. It shows a clear protein peak close to the outer radius and less density in the middle of the oligomer. The number of monomers in the average ring is 50, and the inner radius of the aggregate is approximately 15 nm.

引用

页码：125 / 127

页数：3

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