IDENTIFICATION AND CHARACTERIZATION OF MALE SPECIFIC SERUM-PROTEINS IN THE MEDITERRANEAN FRUIT-FLY CERATITIS-CAPITATA

被引:11
作者
KATSORIS, PG
MAVROIDIS, M
MINTZAS, AC
机构
[1] Division of Genetics, Cell and Developmental Biology, Department of Biology, University of Patras, Patras
来源
INSECT BIOCHEMISTRY | 1990年 / 20卷 / 07期
关键词
C-CAPITATA; MALE SPECIFIC SERUM PROTEINS;
D O I
10.1016/0020-1790(90)90078-9
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Two major and three minor male specific serum proteins (MSSPs) have been identified in the Mediterranean fruit fly Ceratitis capitata. All five MSSps accumulate in the haemolymph during the first 3 days of the adult development and represent more than 50% of the total haemolymph proteins in the adult males. All MSSPs are dimeric proteins consisting of two subunits with molecular weights between 13.5 and 14.5 kDa. MSSP-1, 3, 4 and 5 are homodimers while MSSP-2 is a heterodimer. The two major MSSPs (MSSP-1 and 5) have been isolated. Antisera against these two MSSPs cross-react partially with each other's antigens but did nt give any reaction with haemolymph from adult females and third instar larvae.
引用
收藏
页码:653 / 657
页数:5
相关论文
共 9 条
[1]  
CLEVELAND DW, 1977, J BIOL CHEM, V252, P1102
[2]  
Engelmann F., 1979, Advances in Insect Physiology, V14, P49, DOI 10.1016/S0065-2806(08)60051-X
[3]   VITELLOGENIN AND VITELLIN IN INSECTS [J].
HAGEDORN, HH ;
KUNKEL, JG .
ANNUAL REVIEW OF ENTOMOLOGY, 1979, 24 :475-505
[4]  
KUNKEL JG, 1985, COMPREHENSIVE INSECT, V1, P83
[5]   CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4 [J].
LAEMMLI, UK .
NATURE, 1970, 227 (5259) :680-+
[6]  
LOWRY OH, 1951, J BIOL CHEM, V193, P265
[7]   ISOLATION, CHARACTERIZATION AND IMMUNOLOGICAL PROPERTIES OF CERATITIN-4, A MAJOR HEMOLYMPH PROTEIN OF THE MEDITERRANEAN FRUIT-FLY CERATITIS-CAPITATA [J].
MINTZAS, AC ;
REBOUTSICAS, D .
INSECT BIOCHEMISTRY, 1984, 14 (03) :285-&
[8]   TRANSLATION OF THE MESSENGER-RNAS CODING FOR THE MAJOR HEMOLYMPH-PROTEINS OF CERATITIS-CAPITATA IN CELL-FREE SYSTEM - COMPARISON OF THE TRANSLATABLE MESSENGER-RNA LEVELS TO THE RESPECTIVE BIOSYNTHETIC LEVELS OF THE PROTEINS IN THE FAT-BODY DURING DEVELOPMENT [J].
MINTZAS, AC ;
CHRYSANTHIS, G ;
CHRISTODOULOU, C ;
MARMARAS, VJ .
DEVELOPMENTAL BIOLOGY, 1983, 95 (02) :492-496
[9]   IMMUNOLOGICAL STUDIES OF AMINATION DEFICIENT STRAINS OF NEUROSPORA CRASSA [J].
ROBERTS, DB ;
PATEMAN, JA .
JOURNAL OF GENERAL MICROBIOLOGY, 1964, 34 (02) :295-&