DETECTION OF VIBRIO-ANGUILLARUM ANTIGEN BY THE DOT BLOT ASSAY

被引：41

作者：

CIPRIANO, RC ^{[1
]}

PYLE, JB ^{[1
]}

STARLIPER, CE ^{[1
]}

PYLE, SW ^{[1
]}

机构：

[1] UNIV GEORGIA, GEORGIA COOPERAT FISHERY RES UNIT, ATHENS, GA 30601 USA

来源：

JOURNAL OF WILDLIFE DISEASES | 1985年 / 21卷 / 03期

关键词：

D O I：

10.7589/0090-3558-21.3.211

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

The dot blot assay, modified and adapted for detection of antigens from V. anguillarum in fish tissues, was specific for V. anguillarum and did not react with antigens of V. ordalii, Pseudomonas sp., or Yersinia ruckeri. The blot assay enabled detection of as little as 2.3 ng of a mixture of protein antigens obtained from cell-free extracts of V. anguillarum; it was .apprx. 100 times more sensitive than either the indirect florescent antibody technique or bacterial isolation for detecting V. anguillarum in fish tissues.

引用

页码：211 / 218

页数：8

共 30 条

[1]

AOKI T, 1978, Fish Pathology, V13, P19

[2]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[3] FLUORESCENT-ANTIBODY IDENTIFICATION AND DETECTION OF CORYNEBACTERIUM CAUSING KIDNEY-DISEASE OF SALMONIDS [J].

BULLOCK, GL ;

STUCKEY, HM .

JOURNAL OF THE FISHERIES RESEARCH BOARD OF CANADA, 1975, 32 (11) :2224-2227

[4] AEROMONAS-SALMONICIDA - RELATIONSHIP BETWEEN EXTRACELLULAR GROWTH PRODUCTS AND ISOLATE VIRULENCE [J].

CIPRIANO, RC ;

GRIFFIN, BR ;

LIDGERDING, BC .

CANADIAN JOURNAL OF FISHERIES AND AQUATIC SCIENCES, 1981, 38 (11) :1322-1326

[5]

CISAR J O, 1969, Bulletin of the Wildlife Disease Association, V5, P73

[6]

CONROY DA, 1974, RIV ITAL PISCIC ITTI, V9, P69

[7]

Dixon P. F., 1981, Bulletin of the European Association of Fish Pathologists, V1, P34

[8] FIRST RECORDS OF VIBRIOSIS IN PACIFIC SALMON CULTURED IN CANADA, AND TAXONOMIC STATUS OF RESPONSIBLE BACTERIUM, VIBRIO-ANGUILLARUM [J].

EVELYN, TPT .

JOURNAL OF THE FISHERIES RESEARCH BOARD OF CANADA, 1971, 28 (04) :517-&

[9] IDENTIFICATION AND TREATMENT OF DISEASES IN THE COMMON SOLE (SOLEA-SOLEA L) [J].

FLUCHTER, J .

AQUACULTURE, 1979, 16 (03) :271-274

[10] VIBRIOSIS IN RAINBOW-TROUT, SALMO-GAIRDNERI RICHARDSON, IN FRESH-WATER IN NORTHEASTERN ITALY [J].

GIORGETTI, G ;

CESCHIA, G .

JOURNAL OF FISH DISEASES, 1982, 5 (02) :125-130

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