NARROW (5-50-MU-M ID) OPEN TUBULAR COLUMNS IN LIQUID-CHROMATOGRAPHY USING IMMOBILIZED POLYMETHYL-OCTADECYLSILOXANE AS STATIONARY PHASE

被引：15

作者：

GOHLIN, K

LARSSON, M

机构：

[1] Department of Analytical and Marine Chemistry, Chalmers University of Technology, University of Göteborg, Göteborg

[2] Astra Hässle AB, Mölndal

来源：

JOURNAL OF MICROCOLUMN SEPARATIONS | 1991年 / 3卷 / 06期

关键词：

OPEN TUBULAR COLUMNS; MICRO-LC; MICRO-GC; SILOXANE; POLYMERIC STATIONARY PHASE; REVERSED-PHASE; COATING PROCEDURE; FUSED SILICA;

D O I：

10.1002/mcs.1220030610

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

This paper presents in detail the preparation of highly efficient, stable open tubular (OT) columns for reversed-phase liquid chromatography. Chromatographic properties of 5-50-mu-m i.d. fused silica columns (1.5-5 m in length) are described, and details of the coating procedure are discussed. On-column fluorescence detection was used for the model substituted-anthracene test solutes. Gas chromatography was used as a complementary technique for column characterization. The stationary phase studied, immobilized polymethyloctadecylsiloxane, PMSC18, was chosen because of its more lipophilic character compared to other siloxanes. As expected, a higher retention power was found compared to polydimethylsiloxane coatings. The polymeric stationary phase showed good properties. More than 900,000 plates were achieved in 25 min (k = 0.1) in a 5-mu-m i.d. fused silica OT column. The stationary phase contribution to band broadening was less than 10% in an 11-mu-m i.d. column at a flow rate thirty times u(opt) (k = 1.3).

引用

页码：547 / 556

页数：10

共 31 条

[1]

Knox J.H., Gilbert M.T., J. Chromatogr., 186, (1979)

[2]

Jorgenson J.W., Guthrie E.J., J. Chromatogr., 255, (1983)

[3]

Tijssen R., Bleumer J.P.A., Smit A.L.C., van Kreveld M.E., J. Chromatogr., 218, (1981)

[4]

Krejci M., Tesarik K., Rusek M., Pajurek J., Flow characteristics and technology of capillary columns with inner diameters less than 15 μm in liquid chromatography, Journal of Chromatography A, 218, (1981)

[5]

van Berkel O., Poppe H., Kraak J.C., Chromatographia, 24, (1987)

[6]

Farbrot A., Folestad S., Larsson M., Polymerized stationary phases in 12 to 50 μm open tubular fused silica columns for LC and GC, Journal of High Resolution Chromatography, 9, (1986)

[7]

Folestad S., Josefsson B., Larsson M., J. Chromatogr., 391, (1987)

[8]

Folestad S., Larsson M., J. Chromatogr., 394, (1987)

[9]

Tock P.P.H., Stegeman G., Peerboom R., Poppe H., Kraak J.C., Unger K.K., Chromatographia, 24, (1987)

[10]

Dluzneski P.R., Jorgenson J.W., New method for the preparation of small diameter columns with polymeric stationary phases for open tubular liquid chromatography, Journal of High Resolution Chromatography, 11, (1988)

← 1 2 3 4 →