INVIVO MICRODIALYSIS ESTIMATION OF HISTAMINE IN HUMAN SKIN

被引:62
作者
ANDERSON, C [1 ]
ANDERSSON, T [1 ]
ANDERSSON, RGG [1 ]
机构
[1] LINKOPING UNIV,DEPT PHARMACOL,S-58185 LINKOPING,SWEDEN
来源
SKIN PHARMACOLOGY | 1992年 / 5卷 / 03期
关键词
HISTAMINE; MICRODIALYSIS; SKIN;
D O I
10.1159/000211035
中图分类号
R75 [皮肤病学与性病学];
学科分类号
100206 ;
摘要
Microdialysis, a new bioanalytical sampling technique, enables the measurement of substances in the extracellular space. This study investigates the use of the technique in the in vivo measurement of histamine levels in human skin. Microdialysis probes are equipped at the tip with a semipermeable polycarbonate membrane which permits the passive diffusion of substances. 16 probes were inserted, via a guide, into the skin of the ventral forearm of 8 patients or volunteers. The probe was perfused at a flow of 5 mul/min, with samples being collected at intervals of 10 min and analysed by RIA technique. The mean histamine level in the first 10-min sample following probe insertion was 39.4 nM. The mean histamine value fell with successive 10-min samples (8.8, 4.6, 2.3 nM). An equilibration period of 40 min following probe insertion is suggested for histamine studies, where provocation of the skin is to be performed. Microdialysis appears to be a promising new tool for quantitative and chronological studies of cutaneous inflammatory mediators.
引用
收藏
页码:177 / 183
页数:7
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