ASSIGNMENT OF THE HUMAN PANCREATIC COLIPASE GENE TO CHROMOSOME-6P21.1 TO PTER

被引：18

作者：

DAVIS, RC

XIA, YR

MOHANDAS, T

SCHOTZ, MC

LUSIS, AJ

机构：

[1] UNIV CALIF LOS ANGELES,LOS ANGELES CTY HARBOR MED CTR,DIV MED GENET,TORRANCE,CA 90509

[2] UNIV CALIF LOS ANGELES,DEPT MICROBIOL,LOS ANGELES,CA 90024

[3] UNIV CALIF LOS ANGELES,DEPT MED,LOS ANGELES,CA 90024

[4] UNIV CALIF LOS ANGELES,INST MOLEC BIOL,LOS ANGELES,CA 90024

来源：

GENOMICS | 1991年 / 10卷 / 01期

关键词：

D O I：

10.1016/0888-7543(91)90509-D

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Pancreatic colipase is a 12-kDa polypeptide cofactor for pancreatic lipase (EC 3.1.1.3), an enzyme essential for the absorption of dietary long-chain triglyceride fatty acids. Colipase is thought to anchor lipase noncovalently to the surface of lipid micelles, counteracting the destabilizing influence of intestinal bile salts. Using primers derived from the known amino acid sequence, we have used the polymerase chain reaction to produce a cDNA clone corresponding to the complete coding region of the human procolipase mRNA. Southern blot analysis of genomic DNA from a panel of mouse-human somatic cell hybrids indicated that the colipase gene (CLPS) resides on human chromosome 6. Further analysis of somatic cell hybrids carrying chromosome 6 translocations permitted regional localization of CLPS to the 6p21.1-pter region. © 1991.

引用

页码：262 / 265

页数：4

共 26 条

[1] EFFECT OF A PROTEIN-DEFICIENT DIET (5 P-100 GLUTEN) AND OF BALANCED REFEEDING (15 P-100 CASEIN) ON POTENTIAL LIPASE, COLIPASE-DEPENDENT LIPASE AND PHOSPHOLIPASE-A2 ACTIVITIES .1. IN THE GROWING-RAT PANCREAS [J].