STIMULATION OF ENDOTHELIAL-CELLS BY PROTEASE ACTIVITY IN COMMERCIAL PREPARATIONS OF XANTHINE-OXIDASE

被引:20
作者
AGER, A
WENHAM, DJ
GORDON, JL
机构
[1] MRC CTR, MECHANISMS TUMOUR IMMUNOL, CAMBRIDGE CB2 2QQ, ENGLAND
[2] MRC, CLIN RES CTR, VASC BIOL LAB, HARROW HA1 3UJ, MIDDX, ENGLAND
关键词
D O I
10.1016/0049-3848(84)90311-6
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
The O2 radical generating system of xanthine oxidase plus xanthine, which has been used as a model for the oxidative burst of activated granulocytes, is known to damage endothelium in vivo and in vitro. Effects (inhibited by catalase, and thus associated with the formation of H2O2) on several parameters of endothelial function were observed using a non-commercial preparation of xanthine oxidase. Xanthine oxidase from 2 different commercial sources has additional effects on endothelial morphology and ion flux that are substrate-independent (i.e., produced in the absence of added xanthine) and are attributable to the presence of pancreatin (a crude enzyme mixture used in the commercial preparation of xanthine oxidase from milk). These effects are related to the tryptic activity of pancreatin and extend previous observations on the effects of neutral proteases on endothelial cells. Studies on the effects of commercial xanthine oxidase preparations on endothelial cells must take account of their trypsin-like activity as well as their capacity to generate O2 products.
引用
收藏
页码:43 / 52
页数:10
相关论文
共 23 条
[1]   DIFFERENTIAL-EFFECTS OF HYDROGEN-PEROXIDE ON INDEXES OF ENDOTHELIAL CELL-FUNCTION [J].
AGER, A ;
GORDON, JL .
JOURNAL OF EXPERIMENTAL MEDICINE, 1984, 159 (02) :592-603
[2]  
DEBONO DP, 1977, IMMUNOLOGY, V32, P221
[3]   INCREASE IN MICRO-VASCULAR PERMEABILITY INDUCED BY ENZYMATICALLY GENERATED FREE-RADICALS .1. INVIVO STUDY [J].
DELMAESTRO, RF ;
BJORK, J ;
ARFORS, KE .
MICROVASCULAR RESEARCH, 1981, 22 (03) :239-254
[4]   PLAQUE FORMATION AND ISOLATION OF PURE LINES WITH POLIOMYELITIS VIRUSES [J].
DULBECCO, R ;
VOGT, M .
JOURNAL OF EXPERIMENTAL MEDICINE, 1954, 99 (02) :167-182
[5]   PREPARATION AND PROPERTIES OF 2 NEW CHROMOGENIC SUBSTRATES OF TRYPSIN [J].
ERLANGER, BF ;
COHEN, W ;
KOKOWSKY, N .
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1961, 95 (02) :271-&
[6]   CHEMISTRY OF XANTHINE OXIDASE .9. IMPROVED METHOD OF PREPARING BOVINE MILK ENZYME [J].
GILBERT, DA ;
BERGEL, F .
BIOCHEMICAL JOURNAL, 1964, 90 (02) :350-&
[7]   NEUTROPHIL-MEDIATED ENDOTHELIAL INJURY INVITRO - MECHANISMS OF CELL DETACHMENT [J].
HARLAN, JM ;
KILLEN, PD ;
HARKER, LA ;
STRIKER, GE ;
WRIGHT, DG .
JOURNAL OF CLINICAL INVESTIGATION, 1981, 68 (06) :1394-1403
[9]   A PLATELET AGGREGATING SUBSTANCE CONTAINED IN XANTHINE-OXIDASE PREPARATION FROM COWS MILK [J].
ISHII, H ;
HIRAISHI, S ;
KAZAMA, M .
THROMBOSIS RESEARCH, 1982, 28 (01) :85-92
[10]   INVIVO DAMAGE OF RAT LUNGS BY OXYGEN METABOLITES [J].
JOHNSON, KJ ;
FANTONE, JC ;
KAPLAN, J ;
WARD, PA .
JOURNAL OF CLINICAL INVESTIGATION, 1981, 67 (04) :983-993