EVALUATION OF EFFECTIVENESS OF BIO-GLAS IN MOLECULAR SIEVING OF POLYPEPTIDES IN GUANIDINE-HYDROCHLORIDE

被引：10

作者：

ANSARI, AA ^{[1
]}

MAGE, RG ^{[1
]}

机构：

[1] NIAID, IMMUNOL LAB, BETHESDA, MD 20014 USA

来源：

ANALYTICAL BIOCHEMISTRY | 1976年 / 74卷 / 01期

关键词：

D O I：

10.1016/0003-2697(76)90314-6

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The effectiveness of Bio-Glas 200 and 500 for separation and MW estimation of polypeptide chains in 6 M guanidine hydrochloride was investigated. The distribution coefficients of 10 proteins after reduction and alkylation were determined. The columns gave good calibration curves when pure proteins were passed individually, but the resolving power of Bio-Glas 200 and 500 in this solvent was very poor, apparently because of their high matrix volume and low inner volume.

引用

页码：118 / 125

页数：8

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