Cytotoxicity and enzyme inhibition studies of polyoxometalates and their chitosan nanoassemblies

被引:54
作者
Shaha, Hamid Saeed [1 ]
Al-Oweinib, Rami [2 ]
Haiderb, Ali [2 ]
Kortzb, Ulrich [2 ]
Iqbala, Jamshed [1 ]
机构
[1] COMSATS Inst Informat Technol, Ctr Adv Drug Res, Abbottabad 22060, Pakistan
[2] Jacobs Univ, Sch Sci & Engn, D-28725 Bremen, Germany
关键词
Polyoxometalate; Chitosan; Cytotoxicity; Alkaline phosphatase; Chitosan-polyoxometalate nanoassemblies;
D O I
10.1016/j.toxrep.2014.06.001
中图分类号
R99 [毒物学(毒理学)];
学科分类号
100405 ;
摘要
Polyoxometalates (POMs) have become very significant in biomedical research for their structural diversity which renders them highly active against bacterial, viral and cancer diseases. In this study three different POMs were synthesized and nanoassemblies were made with chitosan (CTS), a natural biodegradable polymer with excellent drug carrier properties. The compounds were tested on two isoenzymes of alkaline phosphatases including tissue specific calf intestine alkaline phosphatase (CIAP) and tissue non-specific alkaline phosphatase (TNAP). Compound [TeW6O24](6-) (TeW6) showed the highest activity (45.4 +/- 11.3 mu M) among tested compounds against TNAP. Similarly, chitosan-[TeW6O24](6-) (CTS-TeW6) was proved to be a potent inhibitor of CIAP with Ki value of 22 +/- 7 nM. A comparative study was made to evaluate their cytotoxic potential against HeLa cells. Among all tested compounds, Chitosan-[NaP5W30O110](14-) (CTS-P5W30) has showed higher percent cytotoxicity (88 +/- 10%) at 10 M when compared with the standard anticancer drug vincristine (72 +/- 7%). The study revealed that selected POMs proved excellent anticancer potential and were equally effective against alkaline phosphatase enzyme, an increased level of which may indicate cancer metastasis. (C) 2014 The Authors. Published by Elsevier Ireland Ltd.
引用
收藏
页码:341 / 352
页数:12
相关论文
共 79 条
[1]   A HETEROPOLYANION WITH FIVEFOLD MOLECULAR SYMMETRY THAT CONTAINS A NONLABILE ENCAPSULATED SODIUM-ION - THE STRUCTURE AND CHEMISTRY OF [NAP5W30O110]14- [J].
ALIZADEH, MH ;
HARMALKER, SP ;
JEANNIN, Y ;
MARTINFRERE, J ;
POPE, MT .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1985, 107 (09) :2662-2669
[2]   Classical and non-classical ruthenium-based anticancer drugs: Towards targeted chemotherapy [J].
Ang, Wee Han ;
Dyson, Paul J. .
EUROPEAN JOURNAL OF INORGANIC CHEMISTRY, 2006, (20) :4003-4018
[3]   Organic Functionalization of Polyoxovanadates: Sb-N Bonds and Charge Control [J].
Antonova, Elena ;
Naether, Christian ;
Koegerler, Paul ;
Bensch, Wolfgang .
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2011, 50 (03) :764-767
[4]   Decavanadate (V10O286-) and oxovanadates: Oxometalates with many biological activities [J].
Aureliano, Manuel ;
Crans, Debbie C. .
JOURNAL OF INORGANIC BIOCHEMISTRY, 2009, 103 (04) :536-546
[5]   Multilayer films made from poly(allylamine) and phosphorous containing polyoxometalates: Focus on the zeta potential [J].
Ball, Vincent ;
Ringwald, Christian ;
Bour, Jerome ;
Michel, Marc ;
Al-Oweini, Rami ;
Kortz, Ulrich .
JOURNAL OF COLLOID AND INTERFACE SCIENCE, 2013, 409 :166-173
[6]   Effects of tungstate in neonatally streptozotocin-induced diabetic rats: Mechanism leading to normalization of glycaemia [J].
Barbera, A ;
FernandezAlvarez, J ;
Truc, A ;
Gomis, R ;
Guinovart, JJ .
DIABETOLOGIA, 1997, 40 (02) :143-149
[7]   Tungstate is an effective antidiabetic agent in streptozotocin-induced diabetic rats:: a long-term study [J].
Barberà, A ;
Gomis, RR ;
Prats, N ;
Rodríguez-Gil, JE ;
Domingo, M ;
Gomis, R ;
Guinovart, JJ .
DIABETOLOGIA, 2001, 44 (04) :507-513
[8]   Ectonucleotidases in Tumor Cells and Tumor-Associated Immune Cells: An Overview [J].
Bergamin, Leticia Scussel ;
Braganhol, Elizandra ;
Zanin, Rafael Fernandes ;
Albano Edelweiss, Maria Isabel ;
Oliveira Battastini, Ana Maria .
JOURNAL OF BIOMEDICINE AND BIOTECHNOLOGY, 2012,
[9]   Incidence of skeletal complications in patients with bone metastatic prostate cancer and hormone refractory disease: Predictive role of bone resorption and formation markers evaluated at baseline [J].
Berruti, A ;
Dogliotti, L ;
Bitossi, R ;
Fasolis, G ;
Gorzegno, G ;
Bellina, M ;
Torta, M ;
Porpiglia, F ;
Fontana, D ;
Angeli, A .
JOURNAL OF UROLOGY, 2000, 164 (04) :1248-1253
[10]  
Borras-Almenar J.J., 2003, POLYOXOMETALATE MOL