CLONING OF A CDNA-ENCODING A PUTATIVE NICOTINIC ACETYLCHOLINE-RECEPTOR SUBUNIT OF THE HUMAN FILARIAL PARASITE ONCHOCERCA-VOLVULUS

被引：13

作者：

AJUH, PM ^{[1
]}

EGWANG, TG ^{[1
]}

机构：

[1] CTR INT RECH MED FRANCEVILLE,DEPT FILARIAL MOLEC BIOL,FRANCEVILLE,GABON

来源：

GENE | 1994年 / 144卷 / 01期

关键词：

RECOMBINANT DNA; BLINDNESS; ANTHELMINTICS; IVERMECTIN; PATHOGEN; NEUROTRANSMITTER;

D O I：

10.1016/0378-1119(94)90216-X

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

A partial cDNA clone encoding a putative nicotinic acetylcholine receptor (ACHR) subunit of the human filarial parasite, Onchocerca volvulus, has been isolated and sequenced. A truncated open reading frame of 1308 bp capable of encoding 436 amino acids with a calculated M(r) of 51209 was identified. The absence of the double cysteine residues necessary for neurotransmitter binding indicates that the cloned ACHR is a non-alpha subunit.

引用

页码：127 / 129

页数：3

共 13 条

[1] CONSTRUCTION OF ONCHOCERCA-VOLVULUS CDNA LIBRARIES AND PARTIAL CHARACTERIZATION OF THE CDNA FOR A MAJOR ANTIGEN [J].

DONELSON, JE ;

DUKE, BOL ;

MOSER, D ;

ZENG, WL ;

ERONDU, NE ;

LUCIUS, R ;

RENZ, A ;

KARAM, M ;

FLORES, GZ .

MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1988, 31 (03) :241-250

[2] MODE OF ACTION OF THE ANTHELMINTICS MORANTEL, PYRANTEL AND LEVAMISOLE ON MUSCLE-CELL MEMBRANE OF THE NEMATODE ASCARIS-SUUM [J].

HARROW, ID ;

GRATION, KAF .

PESTICIDE SCIENCE, 1985, 16 (06) :662-672

[3] EFFECTS OF SELECTED CHOLINERGIC AND ANTICHOLINERGIC DRUGS ON BRUGIA-MALAYI (NEMATODA) [J].

HILLMAN, GR ;

EWERT, A ;

WESTERFIELD, L ;

GRIM, SO .

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-PHARMACOLOGY TOXICOLOGY & ENDOCRINOLOGY, 1983, 74 (02) :299-301

[4]

KAO PN, 1986, J BIOL CHEM, V261, P8085

[5] THROMBIN RECEPTOR - VARIATIONS ON A THEME [J].

LEFKOWITZ, RJ .

NATURE, 1991, 351 (6325) :353-354

[6] LEVAMISOLE-RESISTANT MUTANTS OF THE NEMATODE CAENORHABDITIS-ELEGANS APPEAR TO LACK PHARMACOLOGICAL ACETYLCHOLINE-RECEPTORS [J].

LEWIS, JA ;

WU, CH ;

LEVINE, JH ;

BERG, H .

NEUROSCIENCE, 1980, 5 (06) :967-989

[7] PRIMARY STRUCTURE OF ALPHA-SUBUNIT PRECURSOR OF TORPEDO-CALIFORNICA ACETYLCHOLINE-RECEPTOR DEDUCED FROM CDNA SEQUENCE [J].

NODA, M ;

TAKAHASHI, H ;

TANABE, T ;

TOYOSATO, M ;

FURUTANI, Y ;

HIROSE, T ;

ASAI, M ;

INAYAMA, S ;

MIYATA, T ;

NUMA, S .

NATURE, 1982, 299 (5886) :793-797

[8] STRUCTURAL HOMOLOGY OF TORPEDO-CALIFORNICA ACETYLCHOLINE-RECEPTOR SUBUNITS [J].

NODA, M ;

TAKAHASHI, H ;

TANABE, T ;

TOYOSATO, M ;

KIKYOTANI, S ;

FURUTANI, Y ;

HIROSE, T ;

TAKASHIMA, H ;

INAYAMA, S ;

MIYATA, T ;

NUMA, S .

NATURE, 1983, 302 (5908) :528-532

[9]

ROHER WH, 1988, COMP BIOCH PHYSL C, V91, P517

[10]

Sambrook J., 1989, MOL CLONING LAB MANU

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