HUMAN UROKINASE CONTAINS GALNAC-BETA(1-4)[FUC-ALPHA(1-3)]GLCNAC-BETA(1-2) AS A NOVEL TERMINAL ELEMENT IN N-LINKED CARBOHYDRATE CHAINS

被引：54

作者：

BERGWERFF, AA

THOMASOATES, JE

VANOOSTRUM, J

KAMERLING, JP

VLIEGENTHART, JFG

机构：

[1] UNIV UTRECHT,BIJVDET CTR,DEPT BIOORGAN CHEM,POB 80075,3508 TB UTRECHT,NETHERLANDS

[2] UNIV UTRECHT,DEPT MASS SPECTROMETRY,UTRECHT,NETHERLANDS

[3] CIBA GEIGY AG,DEPT BIOTECHNOL,CH-4002 BASEL,SWITZERLAND

来源：

FEBS LETTERS | 1992年 / 314卷 / 03期

关键词：

UROKINASE; PLASMINOGEN ACTIVATOR; N-LINKED CARBOHYDRATE CHAIN; FAST ATOM BOMBARDMENT MASS SPECTROSCOPY (FAB-MS); H-1 NMR SPECTROSCOPY;

D O I：

10.1016/0014-5793(92)81512-K

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Structural analysis of enzymically released N-linked carbohydrate chains of human urokinase (urinary-type plasminogen activator) by H-1 NMR spectroscopy and FAB-MS demonstrated that the N-linked oligosaccharides on the only N-glycosylation site contain diantennary structures with the novel GalNAcbeta(1-4)[Fucalpha(1-3)]GlcNAcbeta(1-2) element in the upper or the lower branch.

引用

页码：389 / 394

页数：6

共 29 条

[1] MLEV-17-BASED TWO-DIMENSIONAL HOMONUCLEAR MAGNETIZATION TRANSFER SPECTROSCOPY [J].