MOLECULAR-CLONING AND CHARACTERIZATION OF A CA2+ CALMODULIN-INSENSITIVE ADENYLYL CYCLASE FROM RAT-BRAIN

被引:253
作者
FEINSTEIN, PG
SCHRADER, KA
BAKALYAR, HA
TANG, WJ
KRUPINSKI, J
GILMAN, AG
REED, RR
机构
[1] JOHNS HOPKINS UNIV,SCH MED,DEPT MOLEC BIOL & GENET,BALTIMORE,MD 21205
[2] UNIV TEXAS,SW MED CTR,DEPT PHARMACOL,DALLAS,TX 75235
关键词
CYCLIC AMP; GUANINE NUCLEOTIDE-BINDING PROTEIN; SIGNAL TRANSDUCTION;
D O I
10.1073/pnas.88.22.10173
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Biochemical, immunological, and molecular cloning studies have suggested the existence of multiple forms of adenylyl cyclase (EC 4.6.1.1). An adenylyl cyclase cDNA clone (type II) was isolated from a rat brain library and found to encode a protein of 1090 amino acids that was homologous to but distinct from the previously described Ca2+/calmodulin-stimulated adenylyl cyclase from bovine brain. Expression of the type II cDNA in an insect cell line resulted in an increased level of adenylyl cyclase activity that was insensitive to Ca2+/calmodulin. Addition of activated G(s-alpha) protein to type II-containing membranes increased enzyme activity. The mRNA encoding the type II protein was expressed at high levels in brain tissue and at low levels in olfactory epithelium and lung. The existence of multiple adenylyl cyclase enzymes may provide for complex and distinct modes of biochemical regulation of cAMP levels in the brain.
引用
收藏
页码:10173 / 10177
页数:5
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