Growth and regeneration of rice (Oryza sativa L.) callus in salt medium

Effect of NaCl on the growth of rice callus depends on the concentration of NaCl and variety. Lethal concentration is one factor that shows the level of sensitivity of cells (callus) of the poison effect of NaCl. High concentration of NaCl can decrease growth of the callus; low concentrations stimulate the cells to form tolerance mechanisms in order to survive. Effect of NaCl measured based on the value of LC (lethal concentration). This study aims to determine the LC50 values of NaCl, studied its effect on the growth of rice callus and regeneration of the plantlets. The plant material used were mature embryo of rice Ciherang, Inpari 13, Inpara, Pokkali and IR 29. The treatment is concentrations of NaCl (0, 50, 100, 150 and 200 mM)]. Each treatment consisted of 10 replicates with 5 embryogenic calluses per bottle. The results showed the NaCl LC50 value Ciherang is 85, 78, Inpari 13 is 90.91 and Inpara 3 is 91,77, IR29 70, 67. The increasing of NaCl concentration makes the percentage of callus browning increased, inhibited callus growth and decreased ability callus regenerate.