SEPARATION OF PARALYTIC SHELLFISH TOXINS BY REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY, WITH POSTCOLUMN REACTION AND FLUOROMETRIC DETECTION

被引：97

作者：

FRANCO, JM ^{[1
]}

FERNANDEZVILA, P ^{[1
]}

机构：

[1] IEO,CTR OCEANOG VIGO,E-36280 VIGO,SPAIN

来源：

CHROMATOGRAPHIA | 1993年 / 35卷 / 9-12期

关键词：

COLUMN LIQUID CHROMATOGRAPHY; ION PAIRING; PARALYTIC SHELLFISH TOXINS; DINOFLAGELLATE CULTURES; TOXIC MOLLUSKS;

D O I：

10.1007/BF02267925

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

An RP-HPLC method for the separation of shellfish toxins is described, employing three isocratic systems: Octanesulfonic acid in ammonium phosphate buffer plus acetonitrile for the separation of carbamate toxins (neoSTX, dcSTX and STX), octansulfonic acid in ammonium phosphate buffer for the separation of GTXs and tetrabutylammonium in the same buffer for Cs separation. These eluents perform the separation prior to postcolumn reaction and fluorimetric detection. The procedures have been applied to the determination of toxin production in dinoflagellate cultures, field samples of red tides, and toxic mussels.

引用

页码：613 / 620

页数：8

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