EXPRESSION CLONING OF A NA+-INDEPENDENT NEUTRAL AMINO-ACID TRANSPORTER FROM RAT-KIDNEY

被引：177

作者：

TATE, SS

YAN, N

UDENFRIEND, S

机构：

[1] ROCHE INST MOLEC BIOL,ROCHE RES CTR,DEPT NEUROSCI,NUTLEY,NJ 07110

[2] CORNELL UNIV,MED CTR,COLL MED,DEPT BIOCHEM,NEW YORK,NY 10021

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1992年 / 89卷 / 01期

关键词：

XENOPUS-OOCYTES; SYSTEM-L TRANSPORT;

D O I：

10.1073/pnas.89.1.1

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Uptake of long-chain and aromatic neutral amino acids into cells is known to be catalyzed by the Na+-independent system L transporter, which is ubiquitous in animal cells and tissues. We have used a Xenopus oocyte expression system to clone the cDNA of a system L transporter from a rat kidney cDNA library. The 2.3-kilobase cDNA codes for a protein of 683 amino acids. The transporter has four putative membrane-spanning domains and bears no sequence or structural homology to any known animal or bacterial transporter. When transcribed and expressed in Xenopus oocytes, the transporter exhibits many, but not all, of the characteristics of L-system transporters, suggesting that this represents one of several related L-system transporters.

引用

页码：1 / 5

页数：5

共 31 条

[1] THE IDENTIFICATION OF NEUTRAL AMINO-ACID-TRANSPORT SYSTEMS [J].