PRESENCE OF MOLYBDENUM COFACTOR IN SEEDS OF WHEAT AND BARLEY

被引：6
作者：
ALIKULOV, ZA
SCHIEMANN, J
机构：
[1] ACAD SCI GDR, INST GENET & CROP PLANTS, DDR-4325 GATERSLEBEN, GERMANY
[2] ACAD SCI KASSR, INST MOLEC BIOL & BIOCHEM, ALMA ATA 480012, KAZAKHSTAN, USSR
来源：
PLANT SCIENCE | 1985年 / 40卷 / 03期
关键词：
D O I：
10.1016/0168-9452(85)90197-9
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Molybdenum cofactor (Mo-co) was determined in seeds of wheat and barley by its ability to restore nitrate reductase (NR) activity in extracts of nitrate reductase-deficient mutants. Its activity was compared with that of wheat roots and leaves. Conditions for assay of Mo-co from different sources in the persence of molybdate and reduced glutathione (GSH) were optimised. The effect of heat-treatment of cell-free extracts from seeds, roots and leaves was also investigated. Mutant extracts of Neurospora crassa nit-1 and Nicotiana tabacum CnxA68, used as apoprotein source for in vitro complementation, were shown to give comparable results. The Mo-co activity, extracted from wheat and barley seeds, could be separated into two peaks by gel chromatography.
引用
收藏
页码：161 / 165
页数：5
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