DNA TOPOISOMERASE-V IS A RELATIVE OF EUKARYOTIC TOPOISOMERASE-I FROM A HYPERTHERMOPHILIC PROKARYOTE

被引:80
|
作者
SLESAREV, AI
STETTER, KO
LAKE, JA
GELLERT, M
KRAH, R
KOZYAVKIN, SA
机构
[1] RUSSIAN ACAD SCI,INST MOLEC GENET,MOSCOW 123182,RUSSIA
[2] UKRAINIAN ACAD SCI,RE KAVETSKY INST ONCOL & RADIOBIOL,KIEV 252127,UKRAINE
[3] UNIV CALIF LOS ANGELES,DEPT BIOL,LOS ANGELES,CA 90024
[4] UNIV REGENSBURG,LEHRSTUHL MIKROBIOL,W-8400 REGENSBURG,GERMANY
[5] NIDDK,MOLEC BIOL LAB,BETHESDA,MD 20892
关键词
D O I
10.1038/364735a0
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
THE DNA topoisomerases are ubiquitous enzymes that fulfil vital roles in the replication, transcription and recombination of DNA by carrying out DNA-strand passage reactions1-7. Here we characterize a prokaryotic counterpart to the eukaryotic topoisomerase I in the hyperthermophilic methanogen Methanopyrus kandleri8-10. The new enzyme, called topoisomerase V, has the following properties in common with eukaryotic topoisomerase I, which distinguish it from all other known prokaryotic topoisomerases: (1) its activity is Mg2+-independent; (2) it relaxes both negatively and positively supercoiled DNA; (3) it makes a covalent complex with the 3' end of the broken DNA strand; and (4) it is recognized by antibody raised against human topoisomerase I. Eukaryotic-like enzymes have been discovered in some hyperthermophilic prokaryotes, namely the eocytes11 and the extremely thermophilic archaebacteria12, and hyperthermophilic homologues of eukaryotic DNA polymerase-a, transcription factor IIB and DNA ligase13-15 have all been reported. Thus our findings support the idea that some essential parts of the eukaryotic transcription-translation and replication machineries were in place before the emergence of eukaryotes, and that the closest living relatives of eukaryotes may be hyperthermophiles.
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页码:735 / 736
页数:2
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