SYNTHESIS AND CYTOTOXIC ACTIVITY OF A NEW ALKYLATING DERIVATIVE OF DIPYRIDAMOLE

Synthesis of 2,6 - Bis[bis(2-chloroethyl) amino] - 4,8 dipiperidinopyrimido[5,4- d]pyrimidine, a derivative of dipyridamole, was carried out by treating dipyridamole with thionyl chloride. Cytotoxic activity of this compound was assessed using cultured P388 leukaemia cells and HeLa cells. The compound inhibited the colony-forming ability of HeLa cells and showed a cytotoxicity on P388 cells comparable to that of other alkylating drugs (chlorambucil and CCNU).