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CONFORMATION OF 6,7-DIMETHYL-8-RIBITYLLUMAZINE BOUND TO BETA-SUBUNITS OF HEAVY RIBOFLAVIN SYNTHASE - TRANSFERRED NUCLEAR OVERHAUSER EFFECT (TRNOE) STUDIES EMPLOYING OMEGA-1-C-13-FILTERED NOESY INCLUDING A NOVEL TECHNIQUE FOR ZERO QUANTUM SUPPRESSION
被引：9
|作者：
OSCHKINAT, H
SCHOTT, K
BACHER, A
机构：
[1] MAX PLANCK INST BIOCHEM,W-8033 MARTINSRIED,GERMANY
[2] TECH UNIV MUNICH,LEHRSTUHL ORGAN CHEM & BIOCHEM,W-8046 GARCHING,GERMANY
关键词：
HEAVY RIBOFLAVIN SYNTHASE;
LUMAZINE;
TRANSFERRED NUCLEAR OVERHAUSER EFFECT;
DYNAMIC NOE;
D O I：
10.1007/BF02192798
中图分类号：
Q5 [生物化学];
Q7 [分子生物学];
学科分类号：
071010 ;
081704 ;
摘要：
The beta-subunits of heavy riboflavin synthase catalyze the formation of 6,7-dimethyl-8-ribityllumazine. The interaction of the C-13-labelled enzyme product with isolated beta-subunits was studied by transfer NOE measurements using omega-1-C-13-filtered NOESY and C-13-H-1-relayed NOESY. The advantages of these techniques are the removal of residual enzyme signals, the simplification of zero quantum suppression, and the improvement of water suppression which enabled the semiquantitative study of H-1-H-1 distances of the ligand in the bound state. The preferred conformation of the ribityl side chain was calculated on the basis of the measured distances.
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页码：19 / 32
页数：14
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