DIRECT TRANSFER OF MOLYBDOPTERIN COFACTOR TO APONITRATE REDUCTASE FROM A CARRIER PROTEIN IN CHLAMYDOMONAS-REINHARDTII

被引：29

作者：

AGUILAR, M ^{[1
]}

KALAKOUTSKII, K ^{[1
]}

CARDENAS, J ^{[1
]}

FERNANDEZ, E ^{[1
]}

机构：

[1] UNIV CORDOBA,FAC CIENCIAS,DEPT BIOQUIM & BIOL MOLEC,E-14071 CORDOBA,SPAIN

来源：

FEBS LETTERS | 1992年 / 307卷 / 02期

关键词：

MOLYBDENUM COFACTOR; MOLYBDOPTERIN; NITRATE REDUCTASE; XANTHINE OXIDASE;

D O I：

10.1016/0014-5793(92)80758-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A Chlamydomonas reinhardtii molybdenum cofactor (MoCo)-carrier protein (CP), capable of reconstituting nitrate reductase activity with apoprotein from the Neurospora crassa mutant nit-1, was subjected to experiments of diffusion through a dialysis membrane and gel filtration. CP bonded firmly MoCo and did not release it efficiently unless aponitrate reductase was present in the incubation mixture. Stability of MoCo bound to CP against air and heat was very similar to that of free-MoCo released from milk xanthine oxidase. Our data strongly suggest that MoCo is directly transferred from CP to aponitrate reductase to form an active enzyme.

引用

页码：162 / 163

页数：2

共 19 条

[1]

AGUILAR M, 1991, BIOCHIM BIOPHYS ACTA, V1073, P464

[2] CHARACTERIZATION OF MOLYBDENUM COFACTOR FROM ESCHERICHIA-COLI [J].

AMY, NK ;

RAJAGOPALAN, KV .

JOURNAL OF BACTERIOLOGY, 1979, 140 (01) :114-124

[3]

Fernandez E., 1989, Molecular and genetic aspects of nitrate assimilation., P101

[4] OCCURRENCE OF XANTHINE DEHYDROGENASE IN CHLAMYDOMONAS-REINHARDII - A COMMON COFACTOR SHARED BY XANTHINE DEHYDROGENASE AND NITRATE REDUCTASE [J].

FERNANDEZ, E ;

CARDENAS, J .

PLANTA, 1981, 153 (03) :254-257

[5] INDUCTION OF NITRITE REDUCTASE IN NEUROSPORA-CRASSA [J].

GARRETT, RH .

BIOCHIMICA ET BIOPHYSICA ACTA, 1972, 264 (03) :481-+

[6]

Harris EH, 1989, CHLAMYDOMONAS SOURCE

[7] QUANTITATIVE TRANSFER OF THE MOLYBDENUM COFACTOR FROM XANTHINE-OXIDASE AND FROM SULFITE OXIDASE TO THE DEFICIENT ENZYME OF THE NIT-1 MUTANT OF NEUROSPORA-CRASSA TO YIELD ACTIVE NITRATE REDUCTASE [J].

HAWKES, TR ;

BRAY, RC .

BIOCHEMICAL JOURNAL, 1984, 219 (02) :481-493

[8] BIOGENESIS OF MOLYBDENUM COFACTORS [J].

HINTON, SM ;

DEAN, D .

CRITICAL REVIEWS IN MICROBIOLOGY, 1990, 17 (03) :169-188

[9]

Johnson J. L., 1980, MOLYBDENUM MOLYBDENU, P345

[10] MOLYBDOPTERIN GUANINE DINUCLEOTIDE - A MODIFIED FORM OF MOLYBDOPTERIN IDENTIFIED IN THE MOLYBDENUM COFACTOR OF DIMETHYL-SULFOXIDE REDUCTASE FROM RHODOBACTER-SPHAEROIDES FORMA-SPECIALIS-DENITRIFICANS [J].

JOHNSON, JL ;

BASTIAN, NR ;

RAJAGOPALAN, KV .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (08) :3190-3194

← 1 2 →