DETERMINATION OF AMINO-ACIDS IN APPLE EXTRACTS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：25

作者：

GOMIS, DB ^{[1
]}

LOBO, AMP ^{[1
]}

ALVAREZ, MDG ^{[1
]}

ALONSO, JJM ^{[1
]}

机构：

[1] CONSEJERIA AGRICULTURA & PESCA PRINCIPADO ASTURIAS,CTR EXPERIMENTAC AGRARIA,VILLAVICIOSA,SPAIN

来源：

CHROMATOGRAPHIA | 1990年 / 29卷 / 3-4期

关键词：

Amino acids; Apples; Column liquid chromatography; O-phthaldialdehyde-2-mercaptoethanol;

D O I：

10.1007/BF02268703

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A rapid and sensitive method for the simultaneous determination of primary amino acids in apple is described. After sample preparation, amino acids were derivatized with o-phthaldialdehyde/2-mercaptoethanol and separated on a reversed phase column with a gradient of phosphate buffer-tetrahydrofuran-methanol as the mobile phase. Detection was carried out with a fluorescence detector at excitation and emission wavelengths of 340 nm and 425 nm respectively. Recovery studies showed good results for all substances (91-109%) (with coefficients of variation ranging, from 0.1 to 9.0%). This method was applied to the monitoring of amino acids during the ripening of apples. © 1990 Friedr. Vieweg & Sohn Verlagsgesellschaft mbH.

引用

页码：155 / 160

页数：6

共 24 条

[1] Baron A., 1982, SCI ALIMENT, V2, P15
[2] BIDLINGMEYER BA, 1987, J ASSOC OFF ANA CHEM, V70, P241
[3] BURROUGHS L. F., 1957, Journal of the Science of Food and Agriculture, V8, P122, DOI 10.1002/jsfa.2740080304
[4] CASOLI A, 1982, AM J ENOL VITICULT, V33, P135
[5] AUTOMATED OPTIMIZED HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC ANALYSIS OF PRE-COLUMN O-PHTHALDIALDEHYDE-AMINO ACID-DERIVATIVES
CLOETE, C
[J]. JOURNAL OF LIQUID CHROMATOGRAPHY, 1984, 7 (10): : 1979 - 1990
[6] COLAGRANDE O, 1984, CONN VIGNE VIN, V18
[7] DONHAUSER S, 1986, MONATSSCHRIFT BRAUWI, V11, P391
[8] ELKIN RG, 1985, J ASSOC OFF ANA CHEM, V68, P1028
[9] ELKIN RG, 1987, CEREAL CHEM, V8, P170
[10] APPLICATION OF HPLC TO CHARACTERIZATION AND CONTROL OF INDIVIDUAL ACIDS IN APPLE EXTRACTS AND CIDERS
GOMIS, DB
GUTIERREZ, MJM
ALVAREZ, MDG
ALONSO, JJM
[J]. CHROMATOGRAPHIA, 1988, 25 (12) : 1054 - 1058

← 1 2 3 →