CLONING AND EXPRESSION OF AN ISOFORM OF THE RAT MU-OPIOID RECEPTOR (RMOR1B) WHICH DIFFERS IN AGONIST-INDUCED DESENSITIZATION FROM RMOR1

被引：164
作者：
ZIMPRICH, A
SIMON, T
HOLLT, V
机构：
[1] OTTO VONGUERICKE UNIV,DEPT PHARMACOL & TOXICOL,D-39120 MAGDEBURG,GERMANY
[2] UNIV MUNICH,DEPT PHYSIOL,W-8000 MUNICH,GERMANY
来源：
FEBS LETTERS | 1995年 / 359卷 / 2-3期
关键词：
OPIOID RECEPTOR; ALTERNATIVE SPLICING; DESENSITIZATION; CLONING; G PROTEIN-COUPLED RECEPTOR;
D O I：
10.1016/0014-5793(95)00028-8
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A novel rat mu opioid receptor (rMOR1B) has been isolated. It shows identity to the recently published sequence of rMOR1 [Chen, et al., Mel. Pharmacol., 44 (1993) 8-12] up to amino acid 386 and differs only in length and amino acid composition at the very carboxy-terminal tail. Both mu opioid receptor isoforms, when stably expressed in CHO-K1 cells, show similar affinities to opioid compounds and are equally effective in the inhibition of forskolin-induced cAMP formation, Reverse transcription polymerase chain reaction (RT-PCR) revealed that rMOR1B displays a similar distribution as rMOR1 in various rat brain areas, Studies measuring the inhibition of adenylate cyclase in cells that had been pre-exposed to the mu opioid agonist DAMGO indicated that rMOR1B is much more resistant to agonist-induced desensitization than rMOR1.
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收藏
页码：142 / 146
页数：5
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