Conditions for optimal production of active oxygen forms by polymorphonuclear rat leukocytes

Using the procedure of lysis by ammonium chloride followed by differentiation centrifuging, the fraction of cells containing polymorphonuclear leucocytes was isolated from the whole rat blood to stady the conditions of optimal production of oxygen forms was determined from the chemiluminescent cell response in the presence of luminol. It was shown that chemiluminescent cell response depends on the temperature, pH and osmolarity of the medium as well as the on the presence of various concentrations of Ca2+ in the incubation medium. The maximal chemiluminescent response of polymorphonuclear leucocytes was observed at 35-37 degrees C, pH 7,6, osmolarity of 0,15 and Ca2+ concentration of 5-10 mM.