UNUSUAL STRUCTURE OF THE EXOPOLYSACCHARIDE OF RHIZOBIUM-LEGUMINOSARUM BV VICIAE STRAIN-248

被引：25

作者：

CREMERS, HCJC

STEVENS, K

LUGTENBERG, BJJ

WIJFFELMAN, CA

BATLEY, M

REDMOND, JW

BREEDVELD, MW

ZEVENHUIZEN, LPTM

机构：

[1] MACQUARIE UNIV,SCH CHEM,N RYDE,NSW 2109,AUSTRALIA

[2] AGR UNIV WAGENINGEN,DEPT MICROBIOL,6703 CT WAGENINGEN,NETHERLANDS

来源：

CARBOHYDRATE RESEARCH | 1991年 / 218卷

关键词：

D O I：

10.1016/0008-6215(91)84097-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The exopolysaccharide from R. leguminosarum bv. viciae strain 248 differs from those of other Rhizobium strains with similar symbiotic behavior. C-13-N.m.r. spectroscopy of fragments generated by partial hydrolysis, together with methylation analysis and C-13-N.m.r. spectroscopy of the enzymically depolymerised exopolysaccharide, indicated the following nonasaccharide repeating-unit: [GRAPHICS] The locations of the acetyl and 3-hydroxybutanoyl substituents in the exopolysaccharide are assigned provisionally. R. leguminosarum bv. viciae strain 248, cured of its Sym plasmid pRL1JI, synthesised an exopolysaccharide in which the sites and degree of substitution were unchanged. A Tn5 mutant, derived from strain 248 and unable to induce nodules. synthesised small amounts of EPS that lacked galactose.

引用

页码：185 / 200

页数：16

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