UNIVERSAL BETA-GALACTOSIDASE CLONING VECTORS FOR PROMOTER ANALYSIS AND GENE TARGETING

被引：30

作者：

KAESTNER, KH ^{[1
]}

MONTOLIU, L ^{[1
]}

KERN, H ^{[1
]}

THULKE, M ^{[1
]}

SCHUTZ, G ^{[1
]}

机构：

[1] GERMAN CANC RES CTR,DIV MOLEC BIOL CELL 1,D-69120 HEIDELBERG,GERMANY

来源：

GENE | 1994年 / 148卷 / 01期

关键词：

RECOMBINANT DNA; LACZ; GENE FUSIONS; HOMOLOGOUS RECOMBINATION; TRANSFECTION; NEOMYCIN PHOSPHOTRANSFERASE;

D O I：

10.1016/0378-1119(94)90234-8

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Two new plasmid vectors suitable for generating fusions with the lacZ gene have been developed and tested. The vectors can be applied in the analysis of regulatory elements of eukaryotic genes in both transient and stable transfection experiments. In addition, they can be utilized as the backbone of gene targeting vectors, allowing the assessment of the expression pattern of the targeted gene by staining for beta-galactosidase activity.

引用

页码：67 / 70

页数：4

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