LOCATION OF TOLERATED INSERTIONS DELETIONS IN THE STRUCTURE OF THE MALTOSE-BINDING PROTEIN

被引：30

作者：

BETTON, JM

MARTINEAU, P

SAURIN, W

HOFNUNG, M

机构：

[1] Unite de Programmation Moleculaire et de Toxicologie Genetique (CNRS UA1444), Département des Biotechnologies, Institut Pasteur, Paris

来源：

FEBS LETTERS | 1993年 / 325卷 / 1-2期

关键词：

INSERTION DELETION; LINKER INSERTION MUTAGENESIS; MALTOSE BINDING PROTEIN; PROTEIN STRUCTURE;

D O I：

10.1016/0014-5793(93)81409-S

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In a previous study [(1987) J. Mol. Biol. 194, 663-673], we isolated ten insertion/deletion mutants (indels) of the maltose binding protein for which the maltose binding constant was only a little or not at all affected. In this paper, we have localized these mutations in the recently solved three-dimensional structure. Contrary to the general expectation, most of the insertion/deletion modifications occurred within elements of secondary structure. An analysis of the inserted residues for three indels found within alpha helices allowed an interpretation regarding protein structure accommodation to such modifications.

引用

页码：34 / 38

页数：5

共 25 条

[1] 2-CODON INSERTION MUTAGENESIS OF PLASMID GENES BY USING SINGLE-STRANDED HEXAMERIC OLIGONUCLEOTIDES [J].

BARANY, F .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1985, 82 (12) :4202-4206

[2] LINKER MUTAGENESIS IN THE LACZ-GENE OF ESCHERICHIA-COLI YIELDS VARIANTS OF ACTIVE BETA-GALACTOSIDASE [J].

BREUL, A ;

KUCHINKE, W ;

VONWILCKENBERGMANN, B ;

MULLERHILL, B .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1991, 195 (01) :191-194

[3]

BUSSETA B, 1982, BIOCHIM BIOPHYS ACTA, V701, P111

[4] DISSECTION OF FUNCTIONAL DOMAINS OF ADENOVIRUS DNA-POLYMERASE BY LINKER-INSERTION MUTAGENESIS [J].

CHEN, M ;

HORWITZ, MS .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (16) :6116-6120

[5] SILENT AND FUNCTIONAL-CHANGES IN THE PERIPLASMIC MALTOSE-BINDING PROTEIN OF ESCHERICHIA-COLI-K12 .1. TRANSPORT OF MALTOSE [J].

DUPLAY, P ;

SZMELCMAN, S ;

BEDOUELLE, H ;

HOFNUNG, M .

JOURNAL OF MOLECULAR BIOLOGY, 1987, 194 (04) :663-673

[6] THE HYDROPHOBIC MOMENT DETECTS PERIODICITY IN PROTEIN HYDROPHOBICITY [J].

EISENBERG, D ;

WEISS, RM ;

TERWILLIGER, TC .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1984, 81 (01) :140-144

[7]

FREIMUTH PI, 1990, J BIOL CHEM, V265, P896

[8] HOW AMINO-ACID INSERTIONS ARE ALLOWED IN AN ALPHA-HELIX OF T4-LYSOZYME [J].

HEINZ, DW ;

BAASE, WA ;

DAHLQUIST, FW ;

MATTHEWS, BW .

NATURE, 1993, 361 (6412) :561-564

[9]

HOFNUNG M, 1988, B I PASTEUR, V86, P95

[10] DICTIONARY OF PROTEIN SECONDARY STRUCTURE - PATTERN-RECOGNITION OF HYDROGEN-BONDED AND GEOMETRICAL FEATURES [J].

KABSCH, W ;

SANDER, C .

BIOPOLYMERS, 1983, 22 (12) :2577-2637

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