EXTRACELLULAR ATP ACTIVATES RECEPTOR-OPERATED CATION CHANNELS IN MOUSE LACRIMAL ACINAR-CELLS TO PROMOTE CALCIUM INFLUX IN THE ABSENCE OF PHOSPHOINOSITIDE METABOLISM

被引：82
作者：
SASAKI, T [1 ]
GALLACHER, DV [1 ]
机构：
[1] UNIV LIVERPOOL, PHYSIOL LAB,MRC,SECRETORY CONTROL RES GRP,POB 147, BROWNLOW, LIVERPOOL L69 3BX, ENGLAND
来源：
FEBS LETTERS | 1990年 / 264卷 / 01期
基金：
英国惠康基金;
关键词：
Calcium influx; Extracellular ATP; Lacrimal acinar cell; Purinergic receptor; Receptor-operated channel;
D O I：
10.1016/0014-5793(90)80782-E
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
In exocrine acinar cells a variety of neurotransmitters (e.g. acetylcholine) stimulate phosphatidylinositol 4,5-bisphosphate hydrolysis elevating intracellular calcium to activate calcium-dependent membrane currents (outward K+ and inward Cl-). This study shows that in lacrimal acinar cells extracellular application of ATP is also associated with outward and inward current responses; these, however, are not the result of phosphoinositide metabolism. ATP directly activates receptor-operated cation channels which permit influx of Na+ and Ca+ (the inward current). The elevation in [Ca2+], which results is sufficient to activate the outward K+ current. ATP thus promotes Ca+ influx in the absence of phosphoinositide metabolism. © 1990.
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收藏
页码：130 / 134
页数：5
相关论文
共 20 条
[1]   A NOVEL RECEPTOR-OPERATED CA-2+-PERMEABLE CHANNEL ACTIVATED BY ATP IN SMOOTH-MUSCLE [J].
BENHAM, CD ;
TSIEN, RW .
NATURE, 1987, 328 (6127) :275-278
[2]  
BERRIDGE MJ, 1986, J EXP BIOL, V124, P323
[3]  
BERRIE CP, 1989, MOL PHARMACOL, V35, P526
[4]   COMPOUND-48/80 IS A POTENT INHIBITOR OF PHOSPHOLIPASE-C AND A DUAL MODULATOR OF PHOSPHOLIPASE-A2 FROM HUMAN-PLATELET [J].
BRONNER, C ;
WIGGINS, C ;
MONTE, D ;
MARKI, F ;
CAPRON, A ;
LANDRY, Y ;
FRANSON, RC .
BIOCHIMICA ET BIOPHYSICA ACTA, 1987, 920 (03) :301-305
[5]  
Burnstock G, 1980, Prog Biochem Pharmacol, V16, P141
[6]   INOSITOL 1,3,4,5-TETRAKISPHOSPHATE AND INOSITOL 1,4,5-TRISPHOSPHATE ACT BY DIFFERENT MECHANISMS WHEN CONTROLLING CA-2+ IN MOUSE LACRIMAL ACINAR-CELLS [J].
CHANGYA, L ;
GALLACHER, DV ;
IRVINE, RF ;
PETERSEN, OH .
FEBS LETTERS, 1989, 251 (1-2) :43-48
[7]   EXTRACELLULAR ATP ACTIVATES POLYPHOSPHOINOSITIDE BREAKDOWN AND CA-2+ MOBILIZATION IN EHRLICH ASCITES TUMOR-CELLS [J].
DUBYAK, GR .
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1986, 245 (01) :84-95
[8]   THE [CA-2+]I INCREASE INDUCED IN MURINE THYMOCYTES BY EXTRACELLULAR ATP DOES NOT INVOLVE ATP HYDROLYSIS AND IS NOT RELATED TO PHOSPHOINOSITIDE METABOLISM [J].
ELMOATASSIM, C ;
MAURICE, T ;
MANI, JC ;
DORNAND, J .
FEBS LETTERS, 1989, 242 (02) :391-396
[9]   CALCIUM-DEPENDENT CHLORIDE CURRENTS IN ISOLATED CELLS FROM RAT LACRIMAL GLANDS [J].
EVANS, MG ;
MARTY, A .
JOURNAL OF PHYSIOLOGY-LONDON, 1986, 378 :437-460
[10]   ACETYLCHOLINE STIMULATES A CA-2+-DEPENDENT CL- CONDUCTANCE IN MOUSE LACRIMAL ACINAR-CELLS [J].
FINDLAY, I ;
PETERSEN, OH .
PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY, 1985, 403 (03) :328-330
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