Transcriptome profiling for discovery of genes involved in shoot apical meristem and flower development

被引:14
作者
Singh, Vikash K. [1 ]
Jain, Mukesh [1 ]
机构
[1] Natl Inst Plant Genome Res, Funct & Appl Genom Lab, New Delhi 110067, India
关键词
Shoot apical meristem; Flower development; Chickpea; RNA-seq; Differential expression;
D O I
10.1016/j.gdata.2014.06.004
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Flower development is one of the major developmental processes that governs seed setting in angiosperms. However, little is known about the molecular mechanisms underlying flower development in legumes. Employing RNA-seq for various stages of flower development and few vegetative tissues in chickpea, we identified differentially expressed genes in flower tissues/stages in comparison to vegetative tissues, which are related to various biological processes and molecular functions during flower development. Here, we provide details of experimental methods, RNA-seq data (available at Gene Expression Omnibus database under GSE42679) and analysis pipeline published by Singh and colleagues in the Plant Biotechnology Journal (Singh et al., 2013), along with additional analysis for discovery of genes involved in shoot apical meristem (SAM) development. Our data provide a resource for exploring the complex molecular mechanisms underlying SAM and flower development and identification of gene targets for functional and applied genomics in legumes. (C) 2014 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY-NC-ND license.
引用
收藏
页码:135 / 138
页数:4
相关论文
共 14 条
[1]   Differential expression analysis for sequence count data [J].
Anders, Simon ;
Huber, Wolfgang .
GENOME BIOLOGY, 2010, 11 (10)
[2]   The maize Gα gene COMPACT PLANT2 functions in CLAVATA signalling to control shoot meristem size [J].
Bommert, Peter ;
Il Je, Byoung ;
Goldshmidt, Alexander ;
Jackson, David .
NATURE, 2013, 502 (7472) :555-+
[3]   GCR1 can act independently of heterotrimeric G-protein in response to brassinosteroids and gibberellins in Arabidopsis seed germination [J].
Chen, JG ;
Pandey, S ;
Huang, JR ;
Alonso, JM ;
Ecker, JR ;
Assmann, SM ;
Jones, AM .
PLANT PHYSIOLOGY, 2004, 135 (02) :907-915
[4]   Gene Discovery and Tissue-Specific Transcriptome Analysis in Chickpea with Massively Parallel Pyrosequencing and Web Resource Development [J].
Garg, Rohini ;
Patel, Ravi K. ;
Jhanwar, Shalu ;
Priya, Pushp ;
Bhattacharjee, Annapurna ;
Yadav, Gitanjali ;
Bhatia, Sabhyata ;
Chattopadhyay, Debasis ;
Tyagi, Akhilesh K. ;
Jain, Mukesh .
PLANT PHYSIOLOGY, 2011, 156 (04) :1661-1678
[5]   Validation of internal control genes for quantitative gene expression studies in chickpea (Cicer arietinum L.) [J].
Garg, Rohini ;
Sahoo, Annapurna ;
Tyagi, Akhilesh K. ;
Jain, Mukesh .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2010, 396 (02) :283-288
[6]   RETRACTED: G-protein complex mutants are hypersensitive to abscisic acid regulation of germination and postgermination development (Retracted article. See vol. 181, pg. 1393, 2019) [J].
Pandey, S ;
Chen, JG ;
Jones, AM ;
Assmann, SM .
PLANT PHYSIOLOGY, 2006, 141 (01) :243-256
[7]   NGS QC Toolkit: A Toolkit for Quality Control of Next Generation Sequencing Data [J].
Patel, Ravi K. ;
Jain, Mukesh .
PLOS ONE, 2012, 7 (02)
[8]   A global view of transcriptome dynamics during flower development in chickpea by deep sequencing [J].
Singh, Vikash K. ;
Garg, Rohini ;
Jain, Mukesh .
PLANT BIOTECHNOLOGY JOURNAL, 2013, 11 (06) :691-701
[9]   Modulation of cell proliferation by heterotrimeric G protein in Arabidopsis [J].
Ullah, H ;
Chen, JG ;
Young, JC ;
Im, KH ;
Sussman, MR ;
Jones, AM .
SCIENCE, 2001, 292 (5524) :2066-2069
[10]   Auxin at the Shoot Apical Meristem [J].
Vernoux, Teva ;
Besnard, Fabrice ;
Traas, Jan .
COLD SPRING HARBOR PERSPECTIVES IN BIOLOGY, 2010, 2 (04) :a001487