TRANSFORMATION OF WHITE SPRUCE (PICEA-GLAUCA) SOMATIC EMBRYOS BY MICROPROJECTILE BOMBARDMENT

被引：4

作者：

BOMMINENI, VR ^{[1
]}

CHIBBAR, RN ^{[1
]}

DATLA, RSS ^{[1
]}

TSANG, EWT ^{[1
]}

机构：

[1] NATL RES COUNCIL CANADA,INST PLANT BIOTECHNOL,110 GYMNASIUM PL,SASKATOON S7N 0W9,SASKATCHEWAN,CANADA

来源：

PLANT CELL REPORTS | 1993年 / 13卷 / 01期

关键词：

D O I：

暂无

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Cotyledonary somatic embryos of white spruce [Picea glauca (Moench) Voss] were subjected to microprojectile bombardment with a gene construct containing a gus=nptII fusion gene. Somatic embryos were used to re-induce the embryogenic tissue after bombardments. Histochemical assay using X-gluc as a substrate showed that all the embryos (100%) were GUS positive 48 h after bombardment. However, only thirteen out of 605 embryos (2.2%) remained GUS positive after two months in culture. Three of those thirteen (23%) embryo-derived tissues consistently showed GUS activity for eight months in culture. These putatively transformed embryogenic tissues were subjected to Southern blot analysis and the results suggested integration of the gus=nptII gene expression cassette in the white spruce genome.

引用

页码：17 / 23

页数：7

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