TRANSFORMATION OF WHITE SPRUCE (PICEA-GLAUCA) SOMATIC EMBRYOS BY MICROPROJECTILE BOMBARDMENT

被引:4
作者
BOMMINENI, VR [1 ]
CHIBBAR, RN [1 ]
DATLA, RSS [1 ]
TSANG, EWT [1 ]
机构
[1] NATL RES COUNCIL CANADA,INST PLANT BIOTECHNOL,110 GYMNASIUM PL,SASKATOON S7N 0W9,SASKATCHEWAN,CANADA
关键词
D O I
暂无
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Cotyledonary somatic embryos of white spruce [Picea glauca (Moench) Voss] were subjected to microprojectile bombardment with a gene construct containing a gus=nptII fusion gene. Somatic embryos were used to re-induce the embryogenic tissue after bombardments. Histochemical assay using X-gluc as a substrate showed that all the embryos (100%) were GUS positive 48 h after bombardment. However, only thirteen out of 605 embryos (2.2%) remained GUS positive after two months in culture. Three of those thirteen (23%) embryo-derived tissues consistently showed GUS activity for eight months in culture. These putatively transformed embryogenic tissues were subjected to Southern blot analysis and the results suggested integration of the gus=nptII gene expression cassette in the white spruce genome.
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页码:17 / 23
页数:7
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