MAMMALIAN MUSCLE-CELLS BEAR A CELL-AUTONOMOUS, HERITABLE MEMORY OF THEIR ROSTROCAUDAL POSITION

被引:83
作者
DONOGHUE, MJ
MORRISVALERO, R
JOHNSON, YR
MERLIE, JP
SANES, JR
机构
[1] WASHINGTON UNIV, SCH MED, DEPT MOLEC BIOL & PHARMACOL, ST LOUIS, MO 63110 USA
[2] WASHINGTON UNIV, SCH MED, DEPT PEDIAT, ST LOUIS, MO 63110 USA
基金
美国国家卫生研究院;
关键词
D O I
10.1016/0092-8674(92)90119-W
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We previously documented a > 100-fold rostrocaudal gradient of chloramphenicol acetyltransferase (CAT) expression in the muscles of adult mice that bear a myosin light chain-CAT transgene: successively more caudal muscles express successively higher levels of CAT. Here we studied the development and maintenance of this positional information in vitro. CAT levels reflect the rostrocaudal positions of the muscles from which the cells are derived in cultures established from adult muscles, in clones derived from individual adult myogenic (satellite) cells, in cultures prepared from embryonic myoblasts, and in cell lines derived by retrovirus-mediated transfer of an oncogene to satellite cells. Our results suggest that myoblasts bear a positional memory that is established in embryos, retained in adults, cell autonomous, heritable, stable to transformation, and accessible to study in clonal cell lines.
引用
收藏
页码:67 / 77
页数:11
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