THE ENHANCEMENT BY PROSTAGLANDIN-E2 OF CUMULUS CELL OUTGROWTH INVITRO

被引：3

作者：

GOVERDE, HJM

机构：

[1] Laboratory of Endocrinology and Reproduction, Medical Faculty, Nijmegen, Geert Grooteplein zuid 8

来源：

PROSTAGLANDINS | 1993年 / 45卷 / 03期

关键词：

D O I：

10.1016/0090-6980(93)90050-H

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Oocyte-cumulus cell complexes (OCCC) were aspirated from medium-sized (3-5 mm phi) porcine follicles to study quantitatively the process of forming monolayers. A monolayer index (MLI) was therefore developed. This index was evaluated by addition of graded quantities of fetal bovine serum. Furthermore, we observed that addition of prostaglandin E2 accelerated the monolayer formation (P<0.001 at 20 hours versus 40 hours). The effect of PGE2 regarding both monolayer formation and cumulus cell expansion has been discussed.

引用

页码：241 / 247

页数：7

共 20 条

[1] EFFECT OF FSH AND HCG ON PROGESTERONE SECRETION BY CULTURED OOCYTE-CUMULUS COMPLEXES AND GRANULOSA-CELLS FROM PORCINE ANTRAL FOLLICLES - INFLUENCE OF FOLLICULAR DEVELOPMENT [J].