PURIFICATION OF THE CENTROMERE-SPECIFIC PROTEIN CENP-A AND DEMONSTRATION THAT IT IS A DISTINCTIVE HISTONE

被引：325

作者：

PALMER, DK ^{[1
]}

ODAY, K ^{[1
]}

TRONG, HL ^{[1
]}

CHARBONNEAU, H ^{[1
]}

MARGOLIS, RL ^{[1
]}

机构：

[1] UNIV WASHINGTON,DEPT BIOCHEM SJ70,SEATTLE,WA 98195

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1991年 / 88卷 / 09期

关键词：

HISTONE H3; SCLERODERMA; AUTOANTIBODIES; KINETOCHORE; CHROMATIN;

D O I：

10.1073/pnas.88.9.3734

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

CENP-A, a centromere-specific 17-kDa protein, has histone-like properties. However, in contrast to the common somatic histones, CENP-A is quantitatively retained in bull spermatozoa, and we have exploited this fact to purify CENP-A to apparent homogeneity. Partial sequence analysis of the purified protein indicates that CENP-A is a distinctive gene product. Some CENP-A sequences are highly similar to regions of histone H3. Other segments of CENP-A are not related to H3 or any other histone. These unrelated segments are presumably involved in localizing CENP-A to centromeric DNA or in centromere-specific functions of CENP-A.

引用

页码：3734 / 3738

页数：5

共 30 条

[1] INJECTION OF ANTICENTROMERE ANTIBODIES IN INTERPHASE DISRUPTS EVENTS REQUIRED FOR CHROMOSOME MOVEMENT AT MITOSIS [J].