PHOSPHOLIPASE-C FROM CLOSTRIDIUM-PERFRINGENS STIMULATES ACETYLTRANSFERASE-DEPENDENT FORMATION OF PLATELET-ACTIVATING-FACTOR IN CULTURED INTESTINAL EPITHELIAL-CELLS (INT-407)

被引:6
作者
KALD, B
BOLL, RM
GUSTAFSONSVARD, C
SJODAHL, R
TAGESSON, C
机构
[1] LINKOPING UNIV,FAC HLTH SCI,DEPT OCCUPAT & ENVIRONM MED,LINKOPING,SWEDEN
[2] LINKOPING UNIV,FAC HLTH SCI,DEPT MEDICOSURG GASTROENTEROL,LINKOPING,SWEDEN
关键词
ACETYLTRANSFERASE; INFLAMMATION; INTESTINAL EPITHELIAL CELLS; PHOSPHOLIPASE C; PLATELET-ACTIVATION FACTOR-ACETHER;
D O I
10.3109/00365529409090471
中图分类号
R57 [消化系及腹部疾病];
学科分类号
摘要
The mechanisms by which phospholipase C from Clostridium perfringens stimulates the formation of platelet-activating factor (PAF-acether) in cultured intestinal epithelial cells (INT 407) were investigated. Although stimulation with phospholipase C caused a significant formation of PAF-acether, there was no significant increase in the cellular levels of lysoPAF-acether after stimulation. Moreover, when cells prelabeled with H-3-1-O-alkyl-2-acyl-sn-glycerophosphocholine were stimulated with phospholipase C, the H-3-lysoPAF-acether content was not increased in stimulated cells as compared with unstimulated cells. When cells were preincubated with the calmodulin inhibitor trifluoperazine (TFPA), the protein kinase C inhibitor 1-(5-isoquinolinylsulfonyl)-2-methylpiperazine (H-7), or the combined phospholipase A(2)-inhibitor and lipoxygenase inhibitor nordihydroguaiaretic acid (NDGA) before stimulation with phospholipase C, the PAF-acether formation was significantly decreased. The phospholipase AZ inhibitor 4-bromophenacyl bromide (BPB), on the other hand, had no significant effect on the PAF-acether formation. Preincubation with NDGA also decreased the levels of lysoPAF-acether, whereas BPB, H7, or TFPA had no such effect. These findings indicate that stimulation of acetyltransferase activity with increased acetylation of lysoPAF-acether may be one way by which phospholipase C from C. perfringens stimulates formation of PAF-acether in INT 407 cells.
引用
收藏
页码:243 / 247
页数:5
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