INVITRO SHOOT REGENERATION AND CHROMOSOME DOUBLING IN 2X-POTATO AND 3X-POTATO CLONES

被引：16

作者：

CARDI, T ^{[1
]}

CARPUTO, D ^{[1
]}

FRUSCIANTE, L ^{[1
]}

机构：

[1] NAPLES UNIV, DEPT AGRON SCI & PLANT GENET, I-80055 PORTICI, ITALY

来源：

AMERICAN POTATO JOURNAL | 1992年 / 69卷 / 01期

关键词：

SOLANUM-TUBEROSUM; S-CHACOENSE; TISSUE CULTURE; SOMACLONAL VARIATION; POLYPLOIDIZATION;

D O I：

10.1007/BF02853404

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

The possibility of scaling ploidy levels up and down offers several advantages for genetic studies and breeding work in potato. Shoot regeneration and chromosome doubling in plants regenerated from in vitro cultures were investigated in 4 diploid (2n = 2x = 24) and 2 triploid (2n = 3x = 36) clones. Internode and leaf explants were taken from plants propagated either in vitro as shoot cultures, or in vivo in a greenhouse. Two regeneration procedures were compared. Regeneration frequencies were significantly higher using a two-step regeneration protocol and from leaf explants, while doubling was more efficient starting from in vivo grown plants. No doubling was observed in triploid clones. Considering altogether the percentage of regeneration and doubling, and the number of shoots regenerated per explant, the most efficient conditions were considered leaf explants taken from in vivo grown plants, and cultured according to a two-step regeneration protocol.

引用

页码：1 / 12

页数：12

共 23 条

[1] PLANT-REGENERATION FROM INVITRO CULTURES OF STEM INTERNODES IN SELF-INCOMPATIBLE TRIPLOID LYCOPERSICON-PERUVIANUM MILL AND CYTOGENETIC ANALYSIS OF REGENERATED PLANTS [J].

ANCORA, G ;

RAMULU, KS .

PLANT SCIENCE LETTERS, 1981, 22 (03) :197-204

[2]

[Anonymous], 1986, POTATO BREEDING PROB

[3] POLYPLOIDY IN CELL-DIFFERENTIATION [J].

DAMATO, F .

CARYOLOGIA, 1989, 42 (3-4) :183-211

[4] A COMPARISON OF TISSUE-CULTURE RESPONSE BETWEEN RELATED TETRAPLOID AND DIHAPLOID S-TUBEROSUM GENOTYPES [J].

FISH, N ;

JONES, MGK .

PLANT CELL TISSUE AND ORGAN CULTURE, 1988, 15 (03) :201-210

[5] ORIGIN OF CHROMOSOMAL AND PHENOTYPIC VARIATION IN POTATO PROTOCLONES [J].

GILL, BS ;

KAMMORGAN, LNW ;

SHEPARD, JF .

JOURNAL OF HEREDITY, 1986, 77 (01) :13-16

[6] CHROMOSOME DOUBLING THROUGH ADVENTITIOUS SHOOT FORMATION ON INVITRO CULTIVATED LEAF EXPLANTS FROM DIPLOID INTERSPECIFIC POTATO HYBRIDS [J].

HERMSEN, JGT ;

RAMANNA, MS ;

ROEST, S ;

BOKELMANN, GS .

EUPHYTICA, 1981, 30 (02) :239-246

[7]

JACOBSEN E, 1981, Plant Cell Tissue and Organ Culture, V1, P77, DOI 10.1007/BF02318906

[8]

JACOBSEN E, 1978, Z PFLANZENZUCHT, V80, P80

[9] CHROMOSOME DOUBLING IN MONOHAPLOID AND DIHAPLOID POTATOES BY REGENERATION FROM CULTURED LEAF EXPLANTS [J].

KARP, A ;

RISIOTT, R ;

JONES, MGK ;

BRIGHT, SWJ .

PLANT CELL TISSUE AND ORGAN CULTURE, 1984, 3 (04) :363-373

[10] RECONSTITUTION OF DIPLOIDS FROM LEAF TISSUE OF ANTHER-DERIVED HAPLOIDS IN TOBACCO [J].

KASPERBAUER, MJ ;

COLLINS, GB .

CROP SCIENCE, 1972, 12 (01) :98-+

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