CHARACTERIZATION OF WATER-SOLUBLE GLUCURONIDE AND SULFATE CONJUGATES OF AFLATOXIN B-1 .2. STUDIES IN PRIMARY CULTURES OF RAT HEPATOCYTES

被引：11
作者：
WEI, CI [1 ]
HSIEH, DPH [1 ]
机构：
[1] UNIV CALIF DAVIS,DEPT ENVIRONM TOXICOL,DAVIS,CA 95616
来源：
FOOD AND CHEMICAL TOXICOLOGY | 1985年 / 23卷 / 09期
关键词：
D O I：
10.1016/0278-6915(85)90282-0
中图分类号：
TS2 [食品工业];
学科分类号：
0832 ;
摘要：
Aflatoxin B1 and some of its metabolites were released from water-soluble aflatoxin conjugates isolated from rat primary hepatocyte cultures and hydrolysed by enzymes (β-glucuronidase and sulphatase), by acid or by a combination of both treatments. The presence of AFB1 in the hydrolysates was detected on TLC plates, or indicated indirectly by the Ames mutagenicity assay. The aflatoxin conjugates were not mutagenic to Salmonella typhimurium strain TA98 in the presence of rat-liver S-9 mix. However, following enzymatic hydrolysis, the chloroform extract of the hydrolysate was highly mutagenic to the bacteria, indicating the presence of mutagenic AFB1. The conjugates AFB1-glucuronide and AFB1-sulphate are therefore produced from AFB1 in primary cultures of rat hepatocytes. © 1985.
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页码：821 / 825
页数：5
相关论文
共 19 条
[1]   AFLATOXIN-P - NEW AFLATOXIN METABOLITE IN MONKEYS [J].
DALEZIOS, J ;
WOGAN, GN ;
WEINREB, SM .
SCIENCE, 1971, 171 (3971) :584-&
[2]  
DALEZIOS JI, 1972, CANCER RES, V32, P2297
[3]   METABOLISM OF AFLATOXIN-B1 IN CULTURED MOUSE HEPATOCYTES - COMPARISON WITH RAT AND EFFECTS OF CYCLOHEXENE OXIDE AND DIETHYL MALEATE [J].
DECAD, GM ;
DOUGHERTY, KK ;
HSIEH, DPH ;
BYARD, JL .
TOXICOLOGY AND APPLIED PHARMACOLOGY, 1979, 50 (03) :429-436
[4]   MAINTENANCE OF CYTOCHROME-P-450 AND METABOLISM OF AFLATOXIN-B1 IN PRIMARY HEPATOCYTE CULTURES [J].
DECAD, GM ;
HSIEH, DPH ;
BYARD, JL .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1977, 78 (01) :279-287
[5]  
DEGAN GH, 1978, CHEM-BIOL INTERACT, V22, P239
[6]   PRIMARY CULTURES OF ADULT-MOUSE AND RAT HEPATOCYTES FOR STUDYING THE METABOLISM OF FOREIGN CHEMICALS [J].
DOUGHERTY, KK ;
SPILMAN, SD ;
GREEN, CE ;
STEWARD, AR ;
BYARD, JL .
BIOCHEMICAL PHARMACOLOGY, 1980, 29 (15) :2117-2124
[7]   REDUCTION IN BINDING OF [C-14] AFLATOXIN-B1 TO RAT-LIVER MACROMOLECULES BY PHENOBARBITONE PRETREATMENT [J].
GARNER, RC .
BIOCHEMICAL PHARMACOLOGY, 1975, 24 (16) :1553-1556
[8]   THE COMPARATIVE METABOLISM AND TOXIC POTENCY OF AFLATOXIN-B1 AND AFLATOXIN-M1 IN PRIMARY CULTURES OF ADULT-RAT HEPATOCYTES [J].
GREEN, CE ;
RICE, DW ;
HSIEH, DPH ;
BYARD, JL .
FOOD AND CHEMICAL TOXICOLOGY, 1982, 20 (01) :53-60
[9]   METABOLITE DISTRIBUTION AND RATE OF RESIDUE CLEARANCE IN TURKEYS FED A DIET CONTAINING AFLATOXIN-B1 [J].
GREGORY, JF ;
GOLDSTEIN, SL ;
EDDS, GT .
FOOD AND CHEMICAL TOXICOLOGY, 1983, 21 (04) :463-467
[10]   THE EFFECT OF PHENOBARBITAL PRETREATMENT ON THE METABOLISM, COVALENT BINDING, AND CYTO-TOXICITY OF AFLATOXIN-B1 IN PRIMARY CULTURES OF RAT HEPATOCYTES [J].
LOURY, DJ ;
HSIEH, DPH ;
BYARD, JL .
JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH, 1984, 13 (01) :145-159
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