STRUCTURE, ORGANIZATION AND PROMOTER EXPRESSION OF THE ACTIN-ENCODING GENE IN TRICHODERMA-REESEI

被引：29

作者：

MATHEUCCI, E ^{[1
]}

HENRIQUESILVA, F ^{[1
]}

ELGOGARY, S ^{[1
]}

ROSSINI, CHB ^{[1
]}

LEITE, A ^{[1
]}

VERA, JE ^{[1
]}

URIOSTE, JCC ^{[1
]}

CRIVELLARO, O ^{[1
]}

ELDORRY, H ^{[1
]}

机构：

[1] UNIV SAO PAULO,INST CHEM,DEPT BIOCHEM,BR-05508900 SAO PAULO,BRAZIL

来源：

GENE | 1995年 / 161卷 / 01期

基金：

巴西圣保罗研究基金会;

关键词：

HYGROMYCIN B; DOMINANT SELECTABLE MARKER; NUCLEOTIDE SEQUENCE; MOLECULAR EVOLUTION;

D O I：

10.1016/0378-1119(95)00283-C

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The single gene encoding actin (Act) in the cellulolytic filamentous fungus Trichoderma reesei (Ts) has been isolated and characterized. The gene contains five introns located in identical positions when compared to the putative ancestral actin genes (net) present in Thermomyces lamuginosus and Aspergillus nidulans. The 5' untranslated region (UTR) of the gene contains a TATA-like sequence (TAATA), a C + T-rich region and a potential CCAAT motif. This region was used as a homologous promoter to direct expression of hygromycin-B-resistance-encoding gene as a dominant-selectable Tr marker.

引用

页码：103 / 106

页数：4

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