THE SACCHAROMYCES-CEREVISIAE TRANSLATION INITIATION-FACTOR TIF3 AND ITS MAMMALIAN HOMOLOG, ELF-4B, HAVE RNA ANNEALING ACTIVITY

被引：89
作者：
ALTMANN, M
WITTMER, B
METHOT, N
SONENBERG, N
TRACHSEL, H
机构：
[1] MCGILL UNIV,DEPT BIOCHEM,MONTREAL,PQ H3G 1YG,CANADA
[2] MCGILL UNIV,CTR CANC,MONTREAL,PQ H3G 1YG,CANADA
来源：
EMBO JOURNAL | 1995年 / 14卷 / 15期
关键词：
RNA ANNEALING; RNA BINDING PROTEIN; SACCHAROMYCES CEREVISIAE; TRANSLATION INITIATION FACTOR;
D O I：
10.1002/j.1460-2075.1995.tb00051.x
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The Saccharomyces cerevisiae TIF3 gene encodes the yeast homologue of mammalian translation initiation factor eIF-4B. The have added six histidine residues to the C-terminus of Tif3 protein (Tif3-His6p) and purified the tagged protein by affinity chromatography. Tif3-His6p stimulates translation and mRNA binding to ribosomes in a Tif3-dependent in vitro system. Furthermore, it binds to single-stranded RNA and catalyses the annealing of partially complementary RNA strands in vitro. In parallel experiments, RNA annealing activity could also be demonstrated for mammalian eIF-4B. A role for Tif3/eIF-4B and RNA annealing activity in the scanning process is proposed.
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页码：3820 / 3827
页数：8
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