DIFFERENTIAL-SCANNING-CALORIMETRIC STUDY OF THE IRREVERSIBLE THERMAL-DENATURATION OF 8 KDA CYTOTOXIN FROM THE SEA-ANEMONE RADIANTHUS-MACRODACTYLUS

被引：13
作者：
ZHADAN, GG
SHNYROV, VL
机构：
[1] Departamento Bioquimica Biologia Mol, Facultad de Biologia, Universidad de Salamanca, 37007 Salamanca, Avenida del Campo Charro s/n
来源：
BIOCHEMICAL JOURNAL | 1994年 / 299卷
关键词：
D O I：
10.1042/bj2990731
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A differential-scanning-calorimetric study of the thermal denaturation of a sea-anemone (Radianthus macrodactylus) 8 kDa cytolytic toxin was carried out. The calorimetric traces were found to be irreversible and scan-rate-dependent under the experimental conditions employed. Scan-rate-dependent thermograms were explained in terms of a two-state kinetic model N kappa under right arrow D, where kappa is a first-order kinetic constant that changes with temperature as given by the Arrhenius equation, N is the native state of the toxin, and D the denatured one.
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页码：731 / 733
页数：3
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