SEPARATION OF DNS-AMINO ACID AND VITAMINS BY MICELLAR ELECTROKINETIC CHROMATOGRAPHY

被引：35

作者：

ONG, CP ^{[1
]}

NG, CL ^{[1
]}

LEE, HK ^{[1
]}

LI, SFY ^{[1
]}

机构：

[1] NATL UNIV SINGAPORE,DEPT CHEM,SINGAPORE 0511,SINGAPORE

来源：

JOURNAL OF CHROMATOGRAPHY | 1991年 / 559卷 / 1-2期

关键词：

D O I：

10.1016/0021-9673(91)80101-L

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Fifteen Dns-amino acids were analysed by micellar electrokinetic chromatography (MEKC). All the amino acids were satisfactorily separated within ca. 26 min with 40 mM sodium dodecyl sulphate in phosphate-borate buffer (pH 7.56). The migration behaviour of the amino acids at different pH values and SDS concentrations was examined. In addition, attempts were made to separate a mixture of amino acids and vitamins simultaneously in a single run by MEKC. Detection for this mixture was carried out with an on-column fluorescence detection system with the capability of performing multi-wavelength programming.

引用

收藏

页码：537 / 545

页数：9

相关论文

共 13 条

[1] ELECTROKINETIC SEPARATION OF CHIRAL COMPOUNDS [J].

GASSMANN, E ;

KUO, JE ;

ZARE, RN .

SCIENCE, 1985, 230 (4727) :813-814

[2] ELECTROKINETIC RESOLUTION OF AMINO-ACID ENANTIOMERS WITH COPPER(II) ASPARTAME SUPPORT ELECTROLYTE [J].

GOZEL, P ;

GASSMANN, E ;

MICHELSEN, H ;

ZARE, RN .

ANALYTICAL CHEMISTRY, 1987, 59 (01) :44-49

[3] USE OF COMPLEXING AGENTS FOR SELECTIVE SEPARATION IN HIGH-PERFORMANCE CAPILLARY ELECTROPHORESIS - CHIRAL RESOLUTION VIA CYCLODEXTRINS INCORPORATED WITHIN POLYACRYLAMIDE-GEL COLUMNS [J].

GUTTMAN, A ;

PAULUS, A ;

COHEN, AS ;

GRINBERG, N ;

KARGER, BL .

JOURNAL OF CHROMATOGRAPHY, 1988, 448 (01) :41-53

[4]

Hansch C., 1979, SUBSTITUENT CONSTANT

[5] CHARACTERIZATION OF COLUMN PACKINGS IN NORMAL-PHASE LIQUID-CHROMATOGRAPHY .1. RETENTION BEHAVIOR OF FAT-SOLUBLE VITAMINS IN SILICA GEL-BINARY SOLVENT SYSTEMS [J].

HARA, S ;

ANDO, T ;

NAKAYAMA, Y .

JOURNAL OF LIQUID CHROMATOGRAPHY, 1989, 12 (05) :729-738

[6] ZONE ELECTROPHORESIS IN OPEN-TUBULAR GLASS-CAPILLARIES [J].

JORGENSON, JW ;

LUKACS, KD .

ANALYTICAL CHEMISTRY, 1981, 53 (08) :1298-1302

[7] SEPARATION AND DETERMINATION OF THE INGREDIENTS OF A COLD MEDICINE BY MICELLAR ELECTROKINETIC CHROMATOGRAPHY WITH BILE-SALTS [J].

NISHI, H ;

FUKUYAMA, T ;

MATSUO, M ;

TERABE, S .

JOURNAL OF CHROMATOGRAPHY, 1990, 498 (02) :313-323

[8]

ONG CP, 1990, J CHROMATOGR, V516, P263

[9] ELECTROKINETIC SEPARATIONS WITH MICELLAR SOLUTIONS AND OPEN-TUBULAR CAPILLARIES [J].

TERABE, S ;

OTSUKA, K ;

ICHIKAWA, K ;

TSUCHIYA, A ;

ANDO, T .

ANALYTICAL CHEMISTRY, 1984, 56 (01) :111-113

[10] USE OF A FOCUSING CYLINDRICAL LENS FOR INCREASING SENSITIVITY IN THE OPTICAL-DETECTOR OF A CAPILLARY FLOW-THROUGH CELL [J].

TSUDA, T ;

KOBAYASHI, Y .

JOURNAL OF CHROMATOGRAPHY, 1990, 515 :357-361