HAPLOID CULTURE AND UV MUTAGENESIS IN RAPID-CYCLING BRASSICA-NAPUS FOR THE GENERATION OF RESISTANCE TO CHLORSULFURON AND ALTERNARIA-BRASSICICOLA

被引：29

作者：

AHMAD, I ^{[1
]}

DAY, JP ^{[1
]}

MACDONALD, MV ^{[1
]}

INGRAM, DS ^{[1
]}

机构：

[1] UNIV CAMBRIDGE,SCH BOT,DOWNING ST,CAMBRIDGE CB2 3EA,ENGLAND

来源：

ANNALS OF BOTANY | 1991年 / 67卷 / 06期

关键词：

BRASSICA-NAPUS; MICROSPORE CULTURE; UV LIGHT; MUTAGENESIS; SELECTION; DISEASE RESISTANCE; HERBICIDE; ALTERNARIA-BRASSICICOLA;

D O I：

10.1093/oxfordjournals.aob.a088193

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

The effect of ultra violet (UV) irradiation on cultured isolated microspores of rapid cycling Brassica napus was investigated. The microscpores were highly sensitive to UV, with the calculated LD50 being an exposure of 20 s. Viability tests suggested that death of the microspores was not immediate, but occurred during subsequent incubation (7 d). None of the embryos produced following UV-irradiation of microscpores showed gross morphological variation. A large number of regenerants was established from embryoids and grown to flowering. These plants set fertile seed after selfing. The progenies were assessed for resistance to Alternaria brassicicola and a small number showed increased resistance to the pathogen, suggesting the generation of novel heritable resistance to this pathogen. In vitro selection revealed heritable resistance to the herbicide 'Glean' (active ingredient chlorsulfuron). © 1991 Annals of Botany Company.

引用

页码：521 / 525

页数：5

共 15 条

[1]

Connell S. A., 1986, Nuclear techniques and in vitro culture for plant improvement. Proceedings of a symposium organized jointly by the International Atomic Energy Agency and the Food and Agriculture Organization of the United Nations, 19-23 August 1985, Vienna., P309

[2]

FINCHAM JRS, 1979, FUNGAL GENETICS

[3] NUTRIENT REQUIREMENTS OF SUSPENSION CULTURES OF SOYBEAN ROOT CELLS [J].

GAMBORG, OL ;

MILLER, RA ;

OJIMA, K .

EXPERIMENTAL CELL RESEARCH, 1968, 50 (01) :151-+

[4] EVALUATION OF POLLEN VIABILITY BY ENZYMATICALLY INDUCED FLUORESCENCE - INTRACELLULAR HYDROLYSIS OF FLUORESCEIN DIACETATE [J].

HESLOPHARRISON, J ;

HESLOPHA.Y .

STAIN TECHNOLOGY, 1970, 45 (03) :115-+

[5]

Hutabarat D., 1986, Nuclear techniques and in vitroculture for plant improvement., P187

[6] THE BIOLOGICAL EFFECTS OF GAMMA-IRRADIATION ON SECONDARY EMBRYOIDS OF BRASSICA-NAPUS SSP OLEIFERA (METZG) SINSK, WINTER OILSEED RAPE [J].

MACDONALD, MV ;

NEWSHOLME, DM ;

INGRAM, DS .

NEW PHYTOLOGIST, 1988, 110 (02) :255-259

[7] THE ENHANCEMENT OF ANTHER CULTURE EFFICIENCY IN BRASSICA-NAPUS SSP OLEIFERA METZG (SINSK) USING LOW-DOSES OF GAMMA-IRRADIATION [J].

MACDONALD, MV ;

HADWIGER, MA ;

ASLAM, FN ;

INGRAM, DS .

NEW PHYTOLOGIST, 1988, 110 (01) :101-107

[8]

MACDONALD MV, 1989, SCI PLANT B, V12, P22

[9]

MICKE A, 1983, INDUCED MUTATIONS DI, V2, P3

[10] LARGE-SCALE MICROSPORE CULTURE TECHNIQUE FOR MUTATION SELECTION STUDIES IN BRASSICA-NAPUS [J].

POLSONI, L ;

KOTT, LS ;

BEVERSDORF, WD .

CANADIAN JOURNAL OF BOTANY-REVUE CANADIENNE DE BOTANIQUE, 1988, 66 (08) :1681-1685

← 1 2 →