ADENOLYMPHOMA OF THE SALIVARY-GLAND AS REVEALED BY MODERN HISTOCHEMICAL AND IMMUNOMORPHOLOGICAL METHODS

Samples of 32 adenolymphomas and 10 intact salivary glands were studied using histological, histochemical (Grimelius and congo red staining), microscopic and immunohistochemical methods employing polyclonal antibodies to myosin, human carboanhydrase III and monoclonal antibodies to cytokeratin polypeptides No. 8 and 17, epithelial membrane antigen and T- and B-lymphocytes as well as peroxidase-antiperoxidase reaction. Binding of monoclonal antibodies to cytokeratin No. 8 and epithelial membrane antigen was observed in glandular cells of cysts whereas proliferating cells forming <<Sandersen's cushions>> showed binding of polyclonal antibodies to myosin, carboanhydrase Ill and monoclonal antibodies to cytokeratin No. 17. Tumor stroma revealed T- and B-lymphocytes incretory granulocytes and amyloid spaced along the fiber structures. It is inferred that adenolymphoma is a tumor arising exactly in salivary glands. The epithelial component of this tumor forms following tumor transformation of epithelial and myoepithelial cells of the distal part of salivary gland ducts. T- and B-lymphocytes contribute to formation of tumor stroma. Epithelial membrane antigen is an effective marker of the epithelial component of tumor.