COMPARISON OF PREGNANCY RATES FROM TRANSFER OF FRESH VERSUS COOLED, TRANSPORTED EQUINE EMBRYOS

Donor mares of mixed, light-horse breeds, maintained at Colorado State University, provided 104-embryos for immediate transfer (fresh embryos). One hundred and thirty-six additional embryos were collected on various breeding farms in the United States and were shipped to Colorado State University via commercial airlines (cooled embryos). Embryos were harvested 7 d after ovulation, graded, and either transferred into a mare immediately (< 1 h) or placed in Ham's F-10 medium plus 10% fetal calf serum in an atmosphere of 5% CO2, 5% O2, 90% N2 and packaged in a passive cooling unit (Equitainer) for shipment to our laboratory. All embryos were measured and graded just prior to surgical transfer via flank incision into synchronized mares. Recipients had ovulated 1 or 2 d before (+1, +2), on the same day as (0), or 1, 2 or 3 d after (-1, -2, -3) the donor mare. Pregnancy of recipients was determined by ultrasonography on 12, 35, and 50 d after ovulation of the donor. Pregnancy rates at 12, 35, and 50 d were similar for fresh (74, 64, 61%) and cooled embryos (80, 67, 66%), respectively. Overall, embryo size affected (P < 0.05) pregnancy rates at 12, 35 and 50 d. Embryos of Grade 1 (excellent) or 2 resulted in more pregnancies than those of Grade 3 or 4 (poor) embryos. Embryonic losses between 12 and 35 d or between 35 and 50 d were not altered (P > 0.05) by treatment (fresh or cooled) nor by age of the donor mare (P > 0.05), but embryonic losses between 12 and 35 d were greater (P < 0.06) for embryos stored for > 12 h (25%) versus those stored for < 12 h (10%). The duration needed for shipment (< 12 h or > 12 h) of cooled embryos did not alter pregnancy rates at 12 d (P > 0.05). Age of donor mare had no effect (P > 0.05) upon pregnancy rates of cooled or fresh embryos transferred nor on embryo quality. In summary, equine embryos can be cooled to 5-degrees-C and maintained in storage for up to 24 h without decreased fertility, compared with those of embryos transferred in < 1 hour.