Protective role of glibenclamide against nicotinamide-streptozotocin induced nuclear damage in diabetic Wistar rats

被引：37
作者：
Rabbani, Syed Imam [1 ]
Devi, Kshama [1 ]
Khanam, Salma [2 ]
机构：
[1] Al Ameen Coll Pharm, Dept Pharmacol, Opp Lalbagh Main Gate,Hosur Rd, Bangalore 560027, Karnataka, India
[2] Al Ameen Coll Pharm, Dept Pharmacognosy, Bangalore 560027, Karnataka, India
来源：
JOURNAL OF PHARMACOLOGY & PHARMACOTHERAPEUTICS | 2010年 / 1卷 / 01期
关键词：
Antioxidant; glibenclamide; micronucleus test; sperm abnormality;
D O I：
10.4103/0976-500X.64531
中图分类号：
R9 [药学];
学科分类号：
1007 ;
摘要：
Objective: To evaluate the protective effect of glibenclamide against the experimental diabetes-induced nuclear damage in Wistar rats. Materials and Methods: The anti-mutagenic effect of glibenclamide (0.5, 5 and 50 mg/kg, p.o daily for 4 weeks) was evaluated against the nicotinamide (NA)-streptozotocin (STZ) induced type-2 diabetes mellitus using bone marrow micronucleus and sperm abnormalities tests. The antioxidant status was tested by estimating the serum levels of lipid peroxidation (LPO), catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GPx). Results: The results indicated that glibenclamide at 50 mg/kg decreased the frequency of micronuclei in erythrocytes (P < 0.05) and sperm shape abnormality (P < 0.01) besides enhancing the antioxidant status (P < 0.05) in the diabetic rats. However, glibenclamide treatment did not enhance the polychromatic and normochromatic erythrocytes (P/N) ratio and sperm count in the diabetic condition. Conclusion: The observations indicate that the glibenclamide has anti-mutagenic potential which could be related to the antioxidant effect and might also possess anti-proliferative property.
引用
收藏
页码：18 / 23
页数：6
相关论文
共 41 条
[1]  
Abdul M, 2002, ONCOL REP, V9, P961
[2]   Beneficial effect of vitamin E on the metabolic parameters of diabetic rats [J].
Al Shamsi, MS ;
Amin, A ;
Adeghate, E .
MOLECULAR AND CELLULAR BIOCHEMISTRY, 2004, 261 (1-2) :35-42
[3]   Effect of metformin on clastogenic and biochemical changes induced by adriamycin in Swiss albino mice [J].
Aleisa, A. M. ;
Al-Rejaie, S. S. ;
Bakheet, S. A. ;
Al-Bekari, A. M. ;
Al-Shabanah, O. A. ;
Al-Majed, Abdulhakeem ;
Al-Yahya, Abdulaziz A. ;
Qureshi, S. .
MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS, 2007, 634 (1-2) :93-100
[4]   Effect of the sulfonylurea glyburide on superoxide dismutase in streptozotocin-induced diabetic rat muscle [J].
Altan, N ;
Yigit, S ;
Elmali, E ;
Malhatun, E ;
Rota, S ;
Kilic, N .
GENERAL PHARMACOLOGY, 1997, 28 (05) :795-796
[5]   Assessment of genomic instability in normal and diabetic rats treated with metformin [J].
Attia, S. M. ;
Helal, G. K. ;
Alhaider, A. A. .
CHEMICO-BIOLOGICAL INTERACTIONS, 2009, 180 (02) :296-304
[6]   DNA damage and repair in type 2 diabetes mellitus [J].
Blasiak, J ;
Arabski, M ;
Krupa, R ;
Wozniak, K ;
Zadrozny, M ;
Kasznicki, J ;
Zurawska, M ;
Drzewoski, J .
MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS, 2004, 554 (1-2) :297-304
[7]   The effects of the sulfonylurea glyburide on glutathione peroxidase, superoxide dismutase and catalase activities in the heart tissue of streptozotocin-induced diabetic rat [J].
Bukan, N ;
Sancak, B ;
Bilgihan, A ;
Kosova, F ;
Bugdayci, G ;
Altan, N .
METHODS AND FINDINGS IN EXPERIMENTAL AND CLINICAL PHARMACOLOGY, 2004, 26 (07) :519-522
[8]   EFFECTS OF VITAMIN-A ON CYCLOPHOSPHAMIDE MUTAGENICITY INVITRO (AMES TEST) AND INVIVO (MOUSE MICRONUCLEUS TEST) [J].
BUSK, L ;
SJOSTROM, B ;
AHLBORG, UG .
FOOD AND CHEMICAL TOXICOLOGY, 1984, 22 (09) :725-730
[9]   Nitric oxide mediated endothelium-dependent relaxation induced by glibenclamide in rat isolated aorta [J].
Chan, WK ;
Yao, XQ ;
Ko, WH ;
Huang, Y .
CARDIOVASCULAR RESEARCH, 2000, 46 (01) :180-187
[10]   Induction of chromosome aberrations, micronucleus formation and sperm abnormalities in mouse following carbofuran exposure [J].
Chauhan, LKS ;
Pant, N ;
Gupta, SK ;
Srivastava, SP .
MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS, 2000, 465 (1-2) :123-129
12345