Micropropagation of passion fruit from subcultured multiple shoot primordia

被引:17
|
作者
Kawata, K
Ushida, C
Kawai, F
Kanamori, M
Kuriyama, A
机构
[1] Interdisciplinary Research Institute of Environmental Sciences, Kamigyo-ku, Kyoto, 602, Shichihonmatsu, Itsutsuji-dori
关键词
Passiflora edulis; plant regeneration; rejuvenation; root formation;
D O I
10.1016/S0176-1617(11)81520-8
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
A technique for long-term micropropagation of Passiflora edulis from subcultured multiple shoot primordia is described. Excised tissues from leaves cultured on Murashige and Skoog medium (MS medium) supplemented with 3% (w/v) sucrose, 1 mu molL(-1) 6-benzylaminopurine (BA) and 1 mu molL(-1) indole-3-butyric acid (IBA) formed multiple shoot primordia. These shoot primordia were transferred to a medium containing 10 mu M BA and subcultured repeatedly at 3-week intervals. When subcultured shoot primordia were transferred to a medium containing lower concentrations (0.1 or 1.0 mu molL(-1)) of BA, shoots were formed. Subcultured shoot primordia have continued to regenerate plants for more than 3 years. Shoots developed and rooted on MS medium lacking growth regulators. Rooted plants were transferred to pots and grown in a greenhouse. According to the morphology of leaves of regenerated plants, rejuvenation was obtained by this method of in vitro culture.
引用
收藏
页码:281 / 284
页数:4
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