Among 1486 mould strains isolated from natural sources (screened for extracellular glucose oxidase) only 119 (Aspergillus and Penicillium) showed this enzyme activity, As the best glucose oxidase producer, A. niger 0 - 1 was isolated from decaying tree. The dynamics of glucose oxidase synthesis in A. niger 0 - 1 during its culture by submerged method show that the intracellular activity of this enzyme is 10-times higher than its extracellular level. Some properties of the crude glucose oxidase preparation, isolated from the postculture liquids by lyophilization, were examined.