RAPID CONFIRMATION AND REVISION OF THE PRIMARY STRUCTURE OF BOVINE SERUM-ALBUMIN BY ESIMS AND FRIT-FAB LC MS

被引：485
作者：
HIRAYAMA, K
AKASHI, S
FURUYA, M
FUKUHARA, K
机构：
[1] Central Research Laboratories, Ajinomoto Co. Inc., Kawasaki-ku, Kawasaki, Kanagawa, 210
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1990年 / 173卷 / 02期
关键词：
D O I：
10.1016/S0006-291X(05)80083-X
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Incorrectness of the amino acid sequence of bovine serum albumin (BSA) was suggested from the observed molecular weight of BSA obtained by electrospray ionization mass spectrometry (ESIMS). Lack of a tyrosine residue in the position of 156th was found rapidly, by the combination of frit-fast atom bombardment mass spectrometry/liquid chromatography (Frit-FAB LC/MS), automated Edman degradation and tandem mass spectrometry (MS). Then it turned out that BSA is composed of 583 amino acid residues, and that its average molecular weight is not 66267.1, and it is corrected to 66430.3. Moreover the amino acid sequence of the positions of 94th and 95th was corrected to -QE- by using automated Edman degradation method. © 1990 Academic Press, Inc.
引用
收藏
页码：639 / 646
页数：8
相关论文
共 26 条
[1]   A DETERMINATION OF THE POSITIONS OF DISULFIDE BONDS IN PAIM-I, ALPHA-AMYLASE INHIBITOR FROM STREPTOMYCES-CORCHORUSHII, USING FAST ATOM BOMBARDMENT MASS-SPECTROMETRY [J].
AKASHI, S ;
HIRAYAMA, K ;
SEINO, T ;
OZAWA, S ;
FUKUHARA, K ;
OOUCHI, N ;
MURAI, A ;
ARAI, M ;
MURAO, S ;
TANAKA, K ;
NOJIMA, I .
BIOMEDICAL AND ENVIRONMENTAL MASS SPECTROMETRY, 1988, 15 (10) :541-546
[2]   DETERMINATION OF THE PRIMARY STRUCTURE OF PAIM-II, AN ALPHA-AMYLASE INHIBITOR FROM STREPTOMYCES-CORUCHORUSHII, BY HIGH-PERFORMANCE TANDEM MASS-SPECTROMETRY [J].
AKASHI, S ;
HIRAYAMA, K ;
MURAI, A ;
ARAI, M ;
MURAO, S .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1989, 158 (02) :514-519
[3]   TANDEM MASS-SPECTROMETRY APPLIED TO PROTEIN-STRUCTURE PROBLEMS [J].
BIEMANN, K .
BIOCHEMICAL SOCIETY TRANSACTIONS, 1989, 17 (01) :237-243
[4]  
BROWN JR, 1975, FED PROC, V34, P591
[5]  
BROWN JR, 1982, LIPID PROTEIN INTERA, V1, P25
[6]   SURFACE-TENSION OF AMINO-ACID SOLUTIONS - HYDROPHOBICITY SCALE OF AMINO-ACID RESIDUES [J].
BULL, HB ;
BREESE, K .
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1974, 161 (02) :665-670
[7]  
FENG R, 1990, 38TH P ASMS C MASS S
[8]   THE PRIMARY STRUCTURE OF HUMAN EGF PRODUCED BY GENETIC-ENGINEERING, STUDIED BY HIGH-PERFORMANCE TANDEM MASS-SPECTROMETRY [J].
FURUYA, M ;
AKASHI, S ;
HIRAYAMA, K .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1989, 163 (02) :1100-1106
[9]  
GIBSON BW, 1984, J BIOL CHEM, V259, P801
[10]   STRATEGY FOR THE MASS-SPECTROMETRIC VERIFICATION AND CORRECTION OF THE PRIMARY STRUCTURES OF PROTEINS DEDUCED FROM THEIR DNA-SEQUENCES [J].
GIBSON, BW ;
BIEMANN, K .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1984, 81 (07) :1956-1960
123