DIAGNOSIS OF HUMAN IMMUNODEFICIENCY VIRUS-1 INFECTION IN INFANTS - INVITRO PRODUCTION OF VIRUS-SPECIFIC ANTIBODY IN LYMPHOCYTES

In children born to mothers infected with human immunodeficiency virus 1 (HIV-1), a diagnosis of HIV infection cannot be based on a positive antibody result until at least 18 months of age. There is therefore an urgent need for simple and reliable methods ofdiagnosing HIV infection in these infants. The sensitivity and specificity of a test using the invitro antibody production of HIV-specific IgG was assessed in children whose infection status was known and compared with virus and antigen detection. In vitro antibody production sensitivity was 90 to 95%, at least as sensitive as virus culture in antibody-positive infected children. In the first 2 months there is a relatively high proportion of false positive results, possibly a result of contamination by maternal cells. However, after this period in vitro antibody production is a simple, inexpensive and reliable tool for early diagnosis. © 1990 by Williams and Wilkins.