TRYPSIN-LIKE SERINE PROTEINASE ACTION - DETERMINATION OF THE CATALYTIC PARAMETER-KS, PARAMETER-K+2 AND PARAMETER-K+3 UNDER CONDITIONS WHERE THE SUBSTRATE EXCEEDS THE ENZYME CONCENTRATION

被引：7
作者：
ASCENZI, P
MENEGATTI, E
GUARNERI, M
AMICONI, G
机构：
[1] UNIV ROME LA SAPIENZA,DEPT BIOCHEM SCI,CNR,CTR MOLEC BIOL,PIAZZALE ALDO MORO 5,I-00185 ROME,ITALY
[2] UNIV FERRARA,DEPT PHARMACEUT SCI,I-44100 FERRARA,ITALY
来源：
BIOCHIMICA ET BIOPHYSICA ACTA | 1989年 / 998卷 / 02期
关键词：
D O I：
10.1016/0167-4838(89)90275-6
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
引用
收藏
页码：210 / 214
页数：5
相关论文
共 13 条
[1]  
ANTONINI E, 1981, J BIOL CHEM, V256, P2449
[2]   CATALYTIC PROPERTIES OF SERINE PROTEASES .2. COMPARISON BETWEEN HUMAN URINARY KALLIKREIN AND HUMAN UROKINASE, BOVINE BETA-TRYPSIN, BOVINE THROMBIN, AND BOVINE ALPHA-CHYMOTRYPSIN [J].
ASCENZI, P ;
MENEGATTI, E ;
GUARNERI, M ;
BORTOLOTTI, F ;
ANTONINI, E .
BIOCHEMISTRY, 1982, 21 (10) :2483-2490
[3]   ACTIVE-SITE TITRATION OF SERINE PROTEINASES ACTING SELECTIVELY ON CATIONIC SUBSTRATES BY N-ALPHA-CARBOBENZOXY-L-ARGININE PARA-NITROPHENYL ESTER AND N-ALPHA-CARBOBENZOXY-L-LYSINE PARA-NITROPHENYL ESTER - DETERMINATION OF ACTIVE ENZYME CONCENTRATION [J].
ASCENZI, P ;
MENEGATTI, E ;
GUARNERI, M ;
AMICONI, G .
BIOCHIMICA ET BIOPHYSICA ACTA, 1987, 915 (03) :421-425
[4]   THE PH-DEPENDENCE OF PRE-STEADY-STATE AND STEADY-STATE KINETICS FOR THE PORCINE PANCREATIC BETA-KALLIKREIN-B-CATALYZED HYDROLYSIS OF N-ALPHA-CARBOBENZOXY-L-ARGININE PARA-NITROPHENYL ESTER [J].
ASCENZI, P ;
AMICONI, G ;
BOLOGNESI, M ;
GUARNERI, M ;
MENEGATTI, E ;
ANTONINI, E .
BIOCHIMICA ET BIOPHYSICA ACTA, 1984, 785 (1-2) :75-80
[5]   DIFFERENT PRIMARY SPECIFICITY OF PORCINE PANCREATIC BETA-KALLIKREIN-B AND BOVINE BETA-TRYPSIN - A COMPARATIVE STEADY-STATE AND PRE-STEADY-STATE STUDY [J].
ASCENZI, P ;
MENEGATTI, E ;
GUARNERI, M ;
BOLOGNESI, M ;
AMICONI, G .
BIOCHIMICA ET BIOPHYSICA ACTA, 1984, 789 (01) :99-103
[6]  
ASCENZI P, 1983, GAZZ CHIM ITAL, V112, P307
[7]  
Barlow G H, 1976, Methods Enzymol, V45, P239
[8]   DETERMINATION OF CONCENTRATION OF HYDROLYTIC ENZYME SOLUTIONS - ALPHA-CHYMOTRYPSIN TRYPSIN PAPAIN ELASTASE SUBTILISIN AND ACETYLCHOLINESTERASE [J].
BENDER, ML ;
BEGUECAN.ML ;
BLAKELEY, RL ;
BRUBACHER, LJ ;
FEDER, J ;
GUNTER, CR ;
KEZDY, FJ ;
KILLHEFFER, JV ;
MARSHALL, TH ;
MILLER, CG ;
ROESKE, RW ;
STOOPS, JK .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1966, 88 (24) :5890-+
[9]   COMPARISON OF ESTERASE ACTIVITIES OF TRYPSIN PLASMIN AND THROMBIN ON GUANIDINOBENZOATE ESTERS . TITRATION OF ENZYMES [J].
CHASE, T ;
SHAW, E .
BIOCHEMISTRY, 1969, 8 (05) :2212-&
[10]  
FIEDLER F, 1981, METHOD ENZYMOL, V80, P493
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